Anti-GABA-A-R, Alpha1 Antibody FL594 Conjugate (N95/35)

Name: Anti-GABA-A-R, Alpha1 Antibody FL594 Conjugate
Brand: NeuroMab
SKU: 75-136-FL594
Price: 543.0 USD
Availability: InStock

Our mouse GABA(A)R, Alpha1 FL594 conjugate monoclonal antibody from NeuroMab is produced in-house from clone N95/35. It detects human, mouse, rat, and Xenopus GABA(A)R, Alpha1 and is purified by Protein A chromatography. Works in IHC, ICC.

Anti-GABA-A-R, Alpha1 Antibody FL594 Conjugate

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Species Reactivity

Human, Mouse, Rat, Xenopus

Applications

ICC, IHC

Host Species

Mouse

Conjugates and Formulations

Unconjugated

490

550

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-136-FL594

Volume: 200 µL

Variant

Price:

$543.00

Quantity:

Ships: 1-5 business days

Product Details

Target

GABA(A)R, Alpha1

Scientific Background

GABA(A)R, Alpha1 (Gamma-aminobutyric acid receptor subunit alpha-1) makes up two of the five subunits in the membrane bound GABA A receptor, the others being two β2 subunits and a single γ2 subunit. The receptor is a ligand gated chloride ion channel found postsynaptically and binds GABA, the major inhibitory neurotransmitter in the CNS. Mutations in GABA(A)R, Alpha1 have been linked to several types of epilepsy.

Format

Purified by Protein A chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Monoclonal

Clone

N95/35

Isotype

IgG2a

Applications

ICC, IHC

Host Species

Mouse

Gene Name

GABRA1

Molecular Weight

55 kDa

Immunogen

Fusion protein amino acids 355-394 of mouse GABA-AR-Alpha1 (accession number P62812) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat, Xenopus

Antibody Registry ID

AB_2939506

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.

Buffer

PBS with 0.09% azide

Conjugate

FL594 Ex: 594 nm, Em: 615 nm

Specificity

No cross-reactivity against GABA-A-R-Alpha2 or -Alpha3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Synonyms

ECA4; Gamma-aminobutyric acid receptor subunit alpha-1; EIEE19; GABA(A receptor subunit alpha-1; EJM; EJM5; Gaba receptor alpha 1 polypeptide; GABA(A receptor; GABA(A receptor subunit alpha 1; GABA(A receptor subunit alpha-1; GABA(A receptor alpha 1; GABRA 1; GABRα1; Gamma aminobutyric acid (GABA A receptor alpha 1; Gamma aminobutyric acid A receptor alpha 1; Gamma aminobutyric acid receptor subunit alpha 1; Gamma aminobutyric acid type A receptor alphα1 subunit; Gamma-aminobutyric acid receptor subunit alpha-1; GBRα1_HUMAN

UniProt ID

P14867
UniProt Summary: Alpha subunit of the heteropentameric ligand-gated chloride channel gated by Gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Alpha-1/GABRA1-containing GABAARs are largely synaptic. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation. GABAARs function also as histamine receptor where histamine binds at the interface of two neighboring beta subunits and potentiates GABA response. GABAARs containing alpha, beta and epsilon subunits also permit spontaneous chloride channel activity while preserving the structural information required for GABA-gated openings. Alpha-1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection. Together with rho subunits, may also control neuronal and glial GABAergic transmission in the cerebellum.

NCBI Gene ID

2554

Shipping

Shipped on ice packs

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Anti-GABA-A-R, Alpha1 Antibody FL594 Conjugate (N95/35)

SKU: 75-136-FL594

Product Details

Bulk Order Anti-GABA-A-R, Alpha1 Antibody FL594 Conjugate

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