Anti-GABA-A-R, Alpha1 Antibody (N95/35)

Name: Anti-GABA-A-R, Alpha1 Antibody
Brand: NeuroMab
SKU: 75-136
Price: 394.0 USD
Availability: InStock

Our mouse GABA(A)R, Alpha1 monoclonal antibody from NeuroMab is produced in-house from clone N95/35. It detects human, mouse, rat, and Xenopus GABA(A)R, Alpha1 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Immunoblot of adult mouse membranes from wild-type (WT) and GABA-A-R-Alpha1 knockout (KO) mice probed with N95/35 (green) and anti-actin polyclonal (red). Image courtesy of Dominique Arion, Kelly Rogers and David Lewis (University of Pittsburgh).

Adult rat brain immunohistochemistry (with antigen retrieval via pepsin pretreatment).

Representative images demonstrating GABRA1 (red) and GAD (green) immunofluorescence staining in WT and APE1 cKO mouse brain. Image from publication CC-BY-4.0. PMID:36465182

The distribution of both α1 (Cat. 75-136, 1:1000; green) and α6 (red) subunits was investigated on the cerebellar granule cells. Both subunits were each co-stained with the inhibitory GABAergic presynaptic neuronal marker protein vGAT (blue). α6 immunoactivity was present along the neurite and α1 was predominately present in synapses opposing the pre-synaptic vGAT (E), where α6 also often co-localized (indicated by arrow heads, D,F). Image from publication CC-BY-4.0. PMID:37108794

$Western blot of synaptosomal fractions isolated from WT C57BL/6 forebrains showing labeling of PACS2, PACS1, HDAC6, WDR37, AMPAR1, GABAaRα1 (Cat. 75-136, 1:1000), PSD95, and actin. Image from publication CC-BY-4.0. PMID:37848409$

Immunoblot of adult mouse membranes from wild-type (WT) and GABA-A-R-Alpha1 knockout (KO) mice probed with N95/35 (green) and anti-actin polyclonal (red). Image courtesy of Dominique Arion, Kelly Rogers and David Lewis (University of Pittsburgh).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat, Xenopus

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

Trial Size

SKU: 75-136

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GABA(A)R, Alpha1

Scientific Background

GABA(A)R, Alpha1 (Gamma-aminobutyric acid receptor subunit alpha-1) makes up two of the five subunits in the membrane bound GABA A receptor, the others being two β2 subunits and a single γ2 subunit. The receptor is a ligand gated chloride ion channel found postsynaptically and binds GABA, the major inhibitory neurotransmitter in the CNS. Mutations in GABA(A)R, Alpha1 have been linked to several types of epilepsy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N95/35

Isotype

IgG2a

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

GABRA1

Molecular Weight

55 kDa

Immunogen

Fusion protein amino acids 355-394 of mouse GABA-AR-Alpha1 (accession number P62812) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat, Xenopus

Antibody Registry ID

AB_2108811

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.62

Dilution Ranges

WB: 1:500
IHC: 1:500
ICC: 1:250

Conjugate

Unconjugated

Specificity

No cross-reactivity against GABA-A-R-Alpha2 or -Alpha3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

ECA4; Gamma-aminobutyric acid receptor subunit alpha-1; EIEE19; GABA(A receptor subunit alpha-1; EJM; EJM5; Gaba receptor alpha 1 polypeptide; GABA(A receptor; GABA(A receptor subunit alpha 1; GABA(A receptor subunit alpha-1; GABA(A receptor alpha 1; GABRA 1; GABRα1; Gamma aminobutyric acid (GABA A receptor alpha 1; Gamma aminobutyric acid A receptor alpha 1; Gamma aminobutyric acid receptor subunit alpha 1; Gamma aminobutyric acid type A receptor alphα1 subunit; Gamma-aminobutyric acid receptor subunit alpha-1; GBRα1_HUMAN

UniProt ID

P14867
UniProt Summary: Alpha subunit of the heteropentameric ligand-gated chloride channel gated by Gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Alpha-1/GABRA1-containing GABAARs are largely synaptic. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. GABAARs containing alpha-1 and beta-2 or -3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation. GABAARs function also as histamine receptor where histamine binds at the interface of two neighboring beta subunits and potentiates GABA response. GABAARs containing alpha, beta and epsilon subunits also permit spontaneous chloride channel activity while preserving the structural information required for GABA-gated openings. Alpha-1-mediated plasticity in the orbitofrontal cortex regulates context-dependent action selection. Together with rho subunits, may also control neuronal and glial GABAergic transmission in the cerebellum.

NCBI Gene ID

2554

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Anti-GABA-A-R, Alpha1 Antibody (N95/35)

SKU: 75-136

Product Details

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