Anti-Pan-QKI Antibody (N147/6)

Name: Anti-Pan-QKI Antibody
Brand: NeuroMab
SKU: 75-168
Price: 337.0 USD
Availability: InStock

Our mouse Pan-QKI monoclonal antibody from NeuroMab is produced in-house from clone N147/6. It detects human, mouse, and rat Pan-QKI and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)], and cerebellum [HBM(Cb)].

Adult rat hippocampus immunohistochemistry (high and low magnification).

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Flag-tagged QKI-5, QKI-6, QKI-7 and QKI-7b or untagged Kv2.1 plasmids and probed with N147/6 (left) and mouse anti-Flag (right).

Western blot of protein extracted from NKX2.5→GFP UD hESCs and day 8 cardiomyocyte (d8 c.myo) cells either wild-type (WT) or lacking QKI (polyclonal KO ‘pKO’) and probed with antibodies for tubulin (magenta) and panQKI (green). Image from publication CC-BY-4.0. PMID: 35544276

Representative confocal images of colocalisation of EdU+ and Pan-QKI in the dorsal WM of mouse spinal cord. Closed arrows denote colocalised cells, open arrows denote non-colocalised cells. Image from publication CC-BY-4.0. PMID:36336067

Western blot image of human mammary epithelial (HMLE) cells and TGF-beta treated HMLE (mesHMLE) cells that were treated with QKI-5 siRNA knockdown labeled with Anti-pan-QKI (Cat. 75-168, 1:1000). Image from publication, CC-BY-4.0. PMID: 38112323.

Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)], and cerebellum [HBM(Cb)].

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-168

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Pan-QKI

Scientific Background

QKI5, QKI6, QKI7 and QKI7b are alternatively spliced mRNAs from the QKI1 gene. The proteins are RNA-binding and have roles in the regulation of splicing, export and stability of mRNA. They are involved in myelinization.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N147/6

Isotype

IgG2b

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

Qki Qk QKI HKQ Qk1 Qka1

Molecular Weight

36-38 kDa

Immunogen

Fusion protein amino acids 1-341 (full-length) of human QKI-5 (accession number Q96PU8) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2173149

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.95

Dilution Ranges

WB: 1:1000
IHC: 1:100
ICC: 1:100

Conjugate

Unconjugated

Specificity

Cross-reacts with QKI-6, QKI-7 and QKI-7b

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

QKI-5, QKI-6, QKI-7 and QKI-7b Protein quaking (RqkI) Protein quaking (Hqk) (HqkI) Protein quaking (MqkI) (qkI)

UniProt ID

Q96PU8

Shipping

Shipped on ice packs

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Anti-Pan-QKI Antibody (N147/6)

SKU: 75-168

Product Details

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