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Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (MM0271) at 1:1000.Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight® 594.
Immunoblot versus crude cardiac mitochondrial preps from WT, Mfn2 KO Mfn1 KO mice and probed with N111/24 TC supe. Samples courtesy of Dr. Suzanne Hoppins, Dr. Laura Lackner and Dr. Jodi Nunnari, UCDavis.
Anti-Mitofusin-1 Antibody (BSA and Azide free)
Anti-Mitofusin-1 Antibody Biotin Conjugate
Anti-Mitofusin-1 Antibody FL490 Conjugate
Anti-Mitofusin-1 Antibody FL550 Conjugate
Anti-Mitofusin-1 Antibody FL594 Conjugate
Anti-Mitofusin-1 Antibody FL650 Conjugate
Adult rat hippocampus immunohistochemistry.Immunoblot versus crude cardiac mitochondrial preps from WT, Mfn1 KO or Mfn2 KO mice and probed with N153/5 TC supe (top) and Porin antibody (bottom). Data courtesy of Dr. Suzanne Hoppins, Dr. Laura Lackner and Dr. Jodi Nunnari, UCDavis.
Anti-Mitofusin-2 Antibody (BSA and Azide free)
Anti-Mitofusin-2 Antibody Biotin Conjugate
Anti-Mitofusin-2 Antibody FL490 Conjugate
Anti-Mitofusin-2 Antibody FL550 Conjugate
Anti-Mitofusin-2 Antibody FL594 Conjugate
Anti-Mitofusin-2 Antibody FL650 Conjugate
Immunofluorescence of COS7 cells expressing mKate2 or mRuby using chicken anti-mKate2 antibody (green) and mKate2 autofluorescence (red), showing complete overlap in the signal in transfected cells. The cells were stained using goat anti-chicken IgY FITC secondary antibody (Cat. 80-1000-FITC) and mounted with Fluoroshield with DAPI mounting medium (Cat. AR-6501). The DAPI nuclear stain (blue) shows the nuclei of both transfected and untransfected cells. Western blotting of mock, mKate2, or mRuby transfected COS-7 cell lysates with Aves Labs chicken anti-mKate2 antibody (0.2 ug/ml) and detected with anti-chicken HRP secondary antibody (Cat. 80-1000-HRP). Aves Labs chicken anti-mKate2 recognizes exogenous mKate2 as well as mRuby, another fluorescent protein derived from Entacmaea quadricolor in COS-7 cells.
Aves Labs Anti-mKate2 Antibody
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Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe.
Anti-MMACHC Antibody (BSA and Azide free)
Anti-MMACHC Antibody Biotin Conjugate
Anti-MMACHC Antibody FL490 Conjugate
Anti-MMACHC Antibody FL550 Conjugate

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