Products: Primary Antibodies

Anti-TrpM6 Antibody (N470/22)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-464	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-464	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human

Applications:

Target Description:

Transient receptor potential cation channel subfamily M member 6 or TrpM6 is encoded by the gene TRPM6. TrpM6 is a member of the transient receptor potential ion channel family and acts as a serine/threonine-protein kinase. TrpM6 has an essential function in magnesium transport and is involved with magnesium absorption in the gut and kidney. TRPM6 is primarily expressed in the kidney, colon, GI tract and placenta kidney. Mutations in this gene are associated with hypomagnesemia with secondary hypocalcemia.

Immunogen:

Fusion protein amino acids 1258-1480 (cytoplasmic C-terminus) of human TrpM6 (also known as Transient receptor potential cation channel subfamily M member 6, Melastatin-related TRP cation channel 6, Channel kinase 2 and CHAK2, accession number Q9BX84)

Protein Name(s):

TrpM6 (Transient receptor potential cation channel subfamily M member 6) (Melastatin-related TRP cation channel 6) (Channel kinase 2)

Gene Name(s):

TRPM6 CHAK2

Antibody Registry ID:

Purified: AB_2714206
TC Supernatant: AB_2631244

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

240 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.