Products: Primary Antibodies

Anti-Slo1/BKAlpha potassium channel Antibody (L6/60)

from NeuroMab

PRODUCT OPTIONS

★ Trial Size Available ★

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-022	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $335.00
Brand	Catalog # 75-022-020	Option Purified Trial-Size	Volume 20 µL	Concentration 1 mg/mL	Price $119.00
Brand	Catalog # 73-022	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $335.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat Zebrafish

Applications:

IHC IP WB

Target Description:

mSlo maxi K+ channel (Calcium-activated potassium channel subunit alpha-1) is a member of BK channel alpha subunit family and has the particularity to be regulated by both Ca2+ and membrane depolarization.BK channels have a tetrameric structure. Each subunit is the product of the KCNMA1 gene. All modulatory subunits encoded by KCNMB1, KCNMB2, KCNMB3, or KCNMB4 can associate with the tetrameric channel. MSlo plays a major role in controlling cell excitability, which regulates smooth muscle contractions, transmitter release, and innate immune system cell activation. MSlo maxi-K+ channel contributes to repolarization of the membrane potential as it exports K+ out of the cytosol.

Immunogen:

Fusion protein amino acids 690-1196 (cytoplasmic C-terminus) of mouse Slo1 (accession number AAA39746); Human: 99% identityRat: 100% identity; Epitope mapped within amino acids 690-715 by Dr. D. Levy (Univ of Chicago); segment is 100% conserved in mouse, rat, human, horse, dog, pig, cow, Rhesus monkey, rabbit.

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

Slo1 maxi-K+ channel

Gene Name(s):

Kcnma1 Kcnma

Antibody Registry ID:

Purified: AB_2249538
Purified Trial-Size: AB_2249538
TC Supernatant: AB_10698180

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

110-130 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.