Products: Primary Antibodies

Anti-Slo1/BKAlpha potassium channel (L6/60)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-022	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $325.00
Catalog # 75-022-020	Option Purified Trial-Size	Volume 20 µL	Concentration 1 mg/mL	Price $109.00
Catalog # 73-022	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $325.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat Zebrafish

Applications:

IHC IP WB

Immunogen:

Fusion protein amino acids 690-1196 (cytoplasmic C-terminus) of mouse Slo1 (accession number AAA39746)
Human: 99% identityRat: 100% identity
Epitope mapped within amino acids 690-715 by Dr. D. Levy (Univ of Chicago); segment is 100% conserved in mouse, rat, human, horse, dog, pig, cow, Rhesus monkey, rabbit.

Cross Reactivity:

None Reported

Protein Name(s):

Calcium-activated potassium channel subunit alpha-1 (BK channel) (BKCA alpha) (Calcium-activated potassium channel, subfamily M subunit alpha-1) (K(VCA)alpha) (KCa1.1) (Maxi K channel) (MaxiK) (Slo-alpha) (Slo1) (mSlo1) (Slowpoke homolog) (Slo homolog) (mSlo)

Gene Name(s):

Kcnma1 Kcnma

Antibody Registry ID:

Purified: AB_2249538
Purified Trial-Size: AB_2249538
TC Supernatant: AB_10698180

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

110-130 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Martinez-Espinosa PL, Yang C, Gonzalez-Perez V, Xia XM, Lingle CJ. (2014), 'Knockout of the BK β2 subunit abolishes inactivation of BK currents in mouse adrenal chromaffin cells and results in slow-wave burst activity..' The Journal of General Physiology. 10.1085/jgp.201411253.
Whitt JP, Montgomery JR, Meredith AL. (2016), 'BK channel inactivation gates daytime excitability in the circadian clock..' Nature Communications. 10.1038/ncomms10837.
Singh H, Li M, Hall L, Chen S, Sukur S, Lu R, Caputo A, Meredith AL, Stefani E, Toro L. (2016), 'MaxiK channel interactome reveals its interaction with GABA transporter 3 and heat shock protein 60 in the mammalian brain..' Neuroscience. 10.1016/j.neuroscience.2015.12.058.
Manzanares D, Krick S, Baumlin N, Dennis JS, Tyrrell J, Tarran R, Salathe M. (2016), 'Airway Surface Dehydration by Transforming Growth Factor β (TGF-β) in Cystic Fibrosis Is Due to Decreased Function of a Voltage-dependent Potassium Channel and Can Be Rescued by the Drug Pirfenidone..' The Journal of Biological Chemistry. 10.1074/jbc.M115.670885.
Pratt CP, He J, Wang Y, Barth AL, Bruchez MP. (2016), 'Fluorogenic Green-Inside Red-Outside (GIRO) Labeling Approach Reveals Adenylyl Cyclase-Dependent Control of BKα Surface Expression..' Bioconjug Chem.. 10.1021/acs.bioconjchem.5b00409.
Dell'Orco JM, Wasserman AH, Chopra R, Ingram MA, Hu YS, Singh V, Wulff H, Opal P, Orr HT, Shakkottai VG. (2015), 'Neuronal Atrophy Early in Degenerative Ataxia Is a Compensatory Mechanism to Regulate Membrane Excitability..' Journal of Neuroscience. 10.1523/JNEUROSCI.1357-15.2015.
Zhao G, Li T, Brochet DX, Rosenberg PB, Lederer WJ. (2015), 'STIM1 enhances SR Ca2+ content through binding phospholamban in rat ventricular myocytes..' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1423295112.
Liu Y, Huang X, He X, Zhou Y, Jiang X, Chen-Kiang S, Jaffrey SR, Xu G. (2015), 'A novel effect of thalidomide and its analogs: suppression of cereblon ubiquitination enhances ubiquitin ligase function..' The FASEB Journal. 10.1096/fj.15-274050.
Leo MD, Bulley S, Bannister JP, Kuruvilla KP, Narayanan D, Jaggar JH. (2015), 'Angiotensin II stimulates internalization and degradation of arterial myocyte plasma membrane BK channels to induce vasoconstriction..' American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00127.2015.
Hirono M, Ogawa Y, Misono K, Zollinger DR, Trimmer JS, Rasband MN, Misonou H. (2015), 'BK Channels Localize to the Paranodal Junction and Regulate Action Potentials in Myelinated Axons of Cerebellar Purkinje Cells..' Journal of Neuroscience. 10.1523/JNEUROSCI.3778-14.2015.
White RS, Zemen BG, Khan Z, Montgomery JR, Herrera GM, Meredith AL. (2014), 'Evaluation of mouse urinary bladder smooth muscle for diurnal differences in contractile properties..' Frontiers in Pharmacology. 10.3389/fphar.2014.00293.
Cornelius RJ, Wen D, Li H, Yuan Y, Wang-France J, Warner PC, Sansom SC. (2015), 'Low Na, high K diet and the role of aldosterone in BK-mediated K excretion..' PLoS One. 10.1371/journal.pone.0115515.
Tanner MR, Hu X, Huq R, Tajhya RB, Sun L, Khan FS, Laragione T, Horrigan FT, Gulko PS, Beeton C. (2015), 'KCa1.1 inhibition attenuates fibroblast-like synoviocyte invasiveness and ameliorates disease in rat models of rheumatoid arthritis..' Arthritis & Rheumatology. 10.1002/art.38883.
Zhang Z, Li M, Lu R, Alioua A, Stefani E, Toro L. (2014), 'The angiotensin II type 1 receptor (AT1R) closely interacts with large conductance voltage- and Ca2+-activated K+ (BK) channels and inhibits their activity independent of G-protein activation..' The Journal of Biological Chemistry. 10.1074/jbc.M114.595603.
Nourian Z, Li M, Leo MD, Jaggar JH, Braun AP, Hill MA. (2014), 'Large conductance Ca2+-activated K+ channel (BKCa) α-subunit splice variants in resistance arteries from rat cerebral and skeletal muscle vasculature..' PLoS One. 10.1371/journal.pone.0098863.
Li Y, Lorca RA, Ma X, Rhodes A, England SK. (2014), 'BK channels regulate myometrial contraction by modulating nuclear translocation of NF-κB..' Endocrinology. 10.1210/en.2014-1152.
Hayoz S, Bradley V, Boerman EM, Nourian Z, Segal SS, Jackson WF. (2014), 'Aging increases capacitance and spontaneous transient outward current amplitude of smooth muscle cells from murine superior epigastric arteries..' American Journal of Physiology-Heart and Circulatory Physiology. 10.1152/ajpheart.00492.2013.
Samengo I, Currò D, Barrese V, Taglialatela M, Martire M. (2014), 'Large conductance calcium-activated potassium channels: their expression and modulation of glutamate release from nerve terminals isolated from rat trigeminal caudal nucleus and cerebral cortex..' Neurochemical Research. 10.1007/s11064-014-1287-1.
Yi F, Wang H, Chai Q, Wang X, Shen WK, Willis MS, Lee HC, Lu T. (2014), 'Regulation of large conductance Ca2+-activated K+ (BK) channel β1 subunit expression by muscle RING finger protein 1 in diabetic vessels..' The Journal of Biological Chemistry. 10.1074/jbc.M113.520940.
Leo MD, Bannister JP, Narayanan D, Nair A, Grubbs JE, Gabrick KS, Boop FA, Jaggar JH. (2014), 'Dynamic regulation of β1 subunit trafficking controls vascular contractility..' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1317527111.
Lu R, Lukowski R, Sausbier M, Zhang DD, Sisignano M, Schuh CD, Kuner R, Ruth P, Geisslinger G, Schmidtko A. (2014), 'BKCa channels expressed in sensory neurons modulate inflammatory pain in mice..' Pain. 10.1016/j.pain.2013.12.005.
Mannowetz N, Naidoo NM, Choo SA, Smith JF, Lishko PV. (2013), 'Slo1 is the principal potassium channel of human spermatozoa..' Elife. 10.7554/eLife.01009.
van der Lubbe N, Moes AD, Rosenbaek LL, Schoep S, Meima ME, Danser AH, Fenton RA, Zietse R, Hoorn EJ. (2013), 'K+-induced natriuresis is preserved during Na+ depletion and accompanied by inhibition of the Na+-Cl- cotransporter..' Am The Journal of Physiology Renal Physiol.. 10.1152/ajprenal.00201.2013.
Montgomery JR, Meredith AL. (2012), 'Genetic activation of BK currents in vivo generates bidirectional effects on neuronal excitability..' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1205573109.
McFerrin MB, Turner KL, Cuddapah VA, Sontheimer H. (2012), 'Differential role of IK and BK potassium channels as mediators of intrinsic and extrinsic apoptotic cell death..' American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00040.2012.
Föller M, Jaumann M, Dettling J, Saxena A, Pakladok T, Munoz C, Ruth P, Sopjani M, Seebohm G, Rüttiger L, Knipper M, Lang F. (2012), 'AMP-activated protein kinase in BK-channel regulation and protection against hearing loss following acoustic overstimulation..' The FASEB Journal. 10.1096/fj.12-208132.
Manning CF, Bundros AM, Trimmer JS. (2012), 'Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance..' PLoS One. 10.1371/journal.pone.0038313.
Menegola M, Clark E, Trimmer JS. (2012), 'The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice..' Epilepsia. 10.1111/j.1528-1167.2012.03485.x.
Zhang XL, Mok LP, Lee KY, Charbonnet M, Gold MS. (2012), 'Inflammation-induced changes in BK(Ca) currents in cutaneous dorsal root ganglion neurons from the adult rat..' Molecular Pain. 10.1186/1744-8069-8-37.
Tanimoto N, Sothilingam V, Euler T, Ruth P, Seeliger MW, Schubert T. (2012), 'BK channels mediate pathway-specific modulation of visual signals in the in vivo mouse retina..' Journal of Neuroscience. 10.1523/JNEUROSCI.4654-11.2012.
Shruti S, Urban-Ciecko J, Fitzpatrick JA, Brenner R, Bruchez MP, Barth AL. (2012), 'The brain-specific Beta4 subunit downregulates BK channel cell surface expression..' PLoS One. 10.1371/journal.pone.0033429.
Almassy J, Won JH, Begenisich TB, Yule DI. (2012), 'Apical Ca2+-activated potassium channels in mouse parotid acinar cells..' The Journal of General Physiology. 10.1085/jgp.201110718.
Zhang DX, Borbouse L, Gebremedhin D, Mendoza SA, Zinkevich NS, Li R, Gutterman DD. (2012), 'H2O2-induced dilation in human coronary arterioles: role of protein kinase G dimerization and large-conductance Ca2+-activated K+ channel activation..' Circulation Research. 10.1161/CIRCRESAHA.111.258871.
Hu X, Laragione T, Sun L, Koshy S, Jones KR, Ismailov II, Yotnda P, Horrigan FT, Gulko PS, Beeton C (2012), 'KCa1.1 potassium channels regulate key proinflammatory and invasive properties of fibroblast-like synoviocytes in rheumatoid arthritis..' The Journal of Biological Chemistry. 10.1074/jbc.M111.312264.
Soloff MS, Jeng YJ, Izban MG, Sinha M, Luxon BA, Stamnes SJ, England SK. (2011), 'Effects of progesterone treatment on expression of genes involved in uterine quiescence..' Reproductive Sciences. 10.1177/1933719111398150.
Wykes R, Kalmbach A, Eliava M, Waters J. (2012), 'Changes in the physiology of CA1 hippocampal pyramidal neurons in preplaque CRND8 mice..' Neurobiology of Aging. 10.1016/j.neurobiolaging.2011.05.001.
McDermott CM, Schrader LA. (2011), 'Activation of κ opioid receptors increases intrinsic excitability of dentate gyrus granule cells..' The Journal of Physiology. 10.1113/jphysiol.2011.211623.
Li BY, Glazebrook P, Kunze DL, Schild JH. (2011), 'KCa1.1 channel contributes to cell excitability in unmyelinated but not myelinated rat vagal afferents..' American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00278.2010.
Tajima N, Itokazu Y, Korpi ER, Somerharju P, Käkelä R. (2011), 'Activity of BK(Ca) channel is modulated by membrane cholesterol content and association with Na+/K+-ATPase in human melanoma IGR39 cells..' The Journal of Biological Chemistry. 10.1074/jbc.M110.149898.
Wersinger E, McLean WJ, Fuchs PA, Pyott SJ. (2010), 'BK channels mediate cholinergic inhibition of high frequency cochlear hair cells..' PLoS One. 10.1371/journal.pone.0013836.
Mazar J, DeYoung K, Khaitan D, Meister E, Almodovar A, Goydos J, Ray A, Perera RJ. (2010), 'The regulation of miRNA-211 expression and its role in melanoma cell invasiveness..' PLoS One. 10.1371/journal.pone.0013779.
Li M, Tanaka Y, Alioua A, Wu Y, Lu R, Kundu P, Sanchez-Pastor E, Marijic J, Stefani E, Toro L. (2010), 'Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation..' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1002685107.
Ernest NJ, Logsdon NJ, McFerrin MB, Sontheimer H, Spiller SE. (2010), 'Biophysical properties of human medulloblastoma cells..' The Journal of Membrane Biology. 10.1007/s00232-010-9306-x.
Zhao G, Neeb ZP, Leo MD, Pachuau J, Adebiyi A, Ouyang K, Chen J, Jaggar JH. (2010), 'Type 1 IP3 receptors activate BKCa channels via local molecular coupling in arterial smooth muscle cells..' The Journal of General Physiology. 10.1085/jgp.201010453.
Martire M, Barrese V, D'Amico M, Iannotti FA, Pizzarelli R, Samengo I, Viggiano D, Ruth P, Cherubini E, Taglialatela M. (2010), 'Pre-synaptic BK channels selectively control glutamate versus GABA release from cortical and hippocampal nerve terminals..' Journal of Neurochemistry. 10.1111/j.1471-4159.2010.06938.x.
Kim EY, Suh JM, Chiu YH, Dryer SE. (2010), 'Regulation of podocyte BK(Ca) channels by synaptopodin, Rho, and actin microfilaments..' Am The Journal of Physiology Renal Physiol.. 10.1152/ajprenal.00206.2010.
Yan J, Aldrich RW. (2010), 'LRRC26 auxiliary protein allows BK channel activation at resting voltage without calcium..' Nature. 10.1038/nature09162.
Kaufmann WA, Kasugai Y, Ferraguti F, Storm JF. (2010), 'Two distinct pools of large-conductance calcium-activated potassium channels in the somatic plasma membrane of central principal neurons..' Neuroscience. 10.1016/j.neuroscience.2010.05.070.
Holtzclaw JD, Liu L, Grimm PR, Sansom SC. (2010), 'Shear stress-induced volume decrease in C11-MDCK cells by BK-alpha/beta4..' Am The Journal of Physiology Renal Physiol.. 10.1152/ajprenal.00222.2010.
Chen X, Kovalchuk Y, Adelsberger H, Henning HA, Sausbier M, Wietzorrek G, Ruth P, Yarom Y, Konnerth A. (2010), 'Disruption of the olivo-cerebellar circuit by Purkinje neuron-specific ablation of BK channels..' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1001745107.
Kim EY, Chiu YH, Dryer SE. (2009), 'Neph1 regulates steady-state surface expression of Slo1 Ca(2+)-activated K(+) channels: different effects in embryonic neurons and podocytes..' American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00354.2009.
Schweizer FE, Savin D, Luu C, Sultemeier DR, Hoffman LF. (2009), 'Distribution of high-conductance calcium-activated potassium channels in rat vestibular epithelia..' Journal of Comparative Neurology. 10.1002/cne.22148.
Ridgway LD, Kim EY, Dryer SE. (2009), 'MAGI-1 interacts with Slo1 channel proteins and suppresses Slo1 expression on the cell surface..' American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00073.2009.
Kim EY, Alvarez-Baron CP, Dryer SE. (2009), 'Canonical transient receptor potential channel (TRPC)3 and TRPC6 associate with large-conductance Ca2+-activated K+ (BKCa) channels: role in BKCa trafficking to the surface of cultured podocytes..' Molecular Pharmacology. 10.1124/mol.108.051912.
Buniel M, Glazebrook PA, Ramirez-Navarro A, Kunze DL. (2008), 'Distribution of voltage-gated potassium and hyperpolarization-activated channels in sensory afferent fibers in the rat carotid body..' Journal of Comparative Neurology. 10.1002/cne.21796.
Yan J, Olsen JV, Park KS, Li W, Bildl W, Schulte U, Aldrich RW, Fakler B, Trimmer JS. (2008), 'Profiling the phospho-status of the BKCa channel alpha subunit in rat brain reveals unexpected patterns and complexity..' Molecular & Cellular Proteomics. 10.1074/mcp.M800063-MCP200.
Kim EY, Choi KJ, Dryer SE. (2008), 'Nephrin binds to the COOH terminus of a large-conductance Ca2+-activated K+ channel isoform and regulates its expression on the cell surface..' Am The Journal of Physiology Renal Physiol.. 10.1152/ajprenal.00140.2008.
Levy DI, Wanderling S, Biemesderfer D, Goldstein SA. (2008), 'MiRP3 acts as an accessory subunit with the BK potassium channel..' Am The Journal of Physiology Renal Physiol.. 10.1152/ajprenal.00598.2007.
Pacheco Otalora LF, Hernandez EF, Arshadmansab MF, Francisco S, Willis M, Ermolinsky B, Zarei M, Knaus HG, Garrido-Sanabria ER. (2008), 'Down-regulation of BK channel expression in the pilocarpine model of temporal lobe epilepsy..' Brain Research. 10.1016/j.brainres.2008.01.017.
Tian L, Chen L, McClafferty H, Sailer CA, Ruth P, Knaus HG, Shipston MJ. (2006), 'A noncanonical SH3 domain binding motif links BK channels to the actin cytoskeleton via the SH3 adapter cortactin..' The FASEB Journal. 10.1096/fj.06-6152fje.
Xu H, Jackson WF, Fink GD, Galligan JJ. (2006), 'Activation of potassium channels by tempol in arterial smooth muscle cells from normotensive and deoxycorticosterone acetate-salt hypertensive rats..' Hypertension.. 10.1161/01.HYP.0000249511.96555.57.
Misonou H, Menegola M, Buchwalder L, Park EW, Meredith A, Rhodes KJ, Aldrich RW, Trimmer JS. (2006), 'Immunolocalization of the Ca2+-activated K+ channel Slo1 in axons and nerve terminals of mammalian brain and cultured neurons..' Journal of Comparative Neurology. 10.1002/cne.20931.