Our mouse Slo1 maxi-K+ channel FL594 conjugate monoclonal antibody from NeuroMab is produced in-house from clone L6/60. It detects drosophila, human, mouse, non-human primate, pig, rat, sheep, Xenopus, and zebrafish Slo1 maxi-K+ channel and is purified by Protein A chromatography. KO validated. Works in IHC, ICC.
mSlo maxi K+ channel (Calcium-activated potassium channel subunit alpha-1) is a member of BK channel alpha subunit family and has the particularity to be regulated by both Ca2+ and membrane depolarization.BK channels have a tetrameric structure. Each subunit is the product of the KCNMA1 gene. All modulatory subunits encoded by KCNMB1, KCNMB2, KCNMB3, or KCNMB4 can associate with the tetrameric channel. MSlo plays a major role in controlling cell excitability, which regulates smooth muscle contractions, transmitter release, and innate immune system cell activation. MSlo maxi-K+ channel contributes to repolarization of the membrane potential as it exports K+ out of the cytosol.
Purified by Protein A chromatography
Lot dependent: provided at 0.3-0.5 mg/mL
Monoclonal
L6/60
IgG2a
ICC, IHC
Mouse
Kcnma1 Kcnma
110-130 kDa
Fusion protein amino acids 690-1196 (cytoplasmic C-terminus) of mouse Slo1 (accession number AAA39746) produced recombinantly in E. Coli
Mouse
Drosophila, Human, Mouse, Non-Human Primate, Pig, Rat, Sheep, Zebrafish
AB_2939150
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL594 Ex: 594 nm, Em: 615 nm
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
12 months from date of receipt
5730414M22Rik; Calcium-activated potassium channel subunit alpha-1; MaxiK; BK channel; Slo; BKCA alpha; Slo1; Calcium-activated potassium channel; mSlo; K(VCAalpha; mSlo1; KCa1.1; BK channel alpha subunit; Maxi K channel; Slo-alpha; slo homolog; Slowpoke homolog; subfamily M subunit alpha-1
Q08460
UniProt Summary: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). Possibly induces sleep when activated by melatonin and through melatonin receptor MTNR1A-dependent dissociation of G-beta and G-gamma subunits, leading to increased sensitivity to Ca(2+) and reduced synaptic transmission.
KO Validated