Products: Primary Antibodies

Anti-Pan-GRK Antibody (N145/20)

from NeuroMab

Product Summary:

This antibody recognizes an epitope common to G protein-coupled receptor kinase 5 (GRK5) and G protein-coupled receptor kinase 6 (GRK6).

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-179	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-179	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N145/20 (left) or N52A/42 (right) TC supe.$

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Target Description:

G protein-coupled receptor (GPCR) kinases (GRKs), are important regulators of GPCR function and mediate receptor desensitization, internalization, and signaling. Several non-GPCR proteins also serve as GRK substrates, and GRKs have been shown to have kinase-independent functions through regulation of receptors and effectors. These proteins are members of the G protein-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. GRKs phosphorylate activated forms of G protein-coupled receptors to regulate their signaling.

Immunogen:

Fusion protein amino acids 381-590 (C-terminus) of mouse GRK5 (also known as G protein-coupled receptor kinase 5 and Gprk5, accession number Q8VEB1); Rat: 99% identity (208/210 amino acids identical); Human: 91% identity (193/210 amino acids identical); Greater than 55% identity with GRK6 and GRK4; Greater than 40% identity with GRK7 and RK

Cross Reactivity:

Cross-reacts with GRK6

Protein Name(s):

Pan-GRK

Gene Name(s):

Grk5 Gprk5 Grk6 Gprk6

Antibody Registry ID:

Purified: AB_2232788
TC Supernatant: AB_10671656

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

70 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.