Products: Primary Antibodies

Anti-Pan-GRK Antibody (N145/20)

NeuroMab Logo from NeuroMab

 Datasheet

Product Summary:

This antibody recognizes an epitope common to G protein-coupled receptor kinase 5 (GRK5) and G protein-coupled receptor kinase 6 (GRK6).

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-179
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-179
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC WB

Target Description:

G protein-coupled receptor (GPCR) kinases (GRKs), are important regulators of GPCR function and mediate receptor desensitization, internalization, and signaling. Several non-GPCR proteins also serve as GRK substrates, and GRKs have been shown to have kinase-independent functions through regulation of receptors and effectors. These proteins are members of the G protein-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. GRKs phosphorylate activated forms of G protein-coupled receptors to regulate their signaling.

Immunogen:

Fusion protein amino acids 381-590 (C-terminus) of mouse GRK5 (also known as G protein-coupled receptor kinase 5 and Gprk5, accession number Q8VEB1); Rat: 99% identity (208/210 amino acids identical); Human: 91% identity (193/210 amino acids identical); Greater than 55% identity with GRK6 and GRK4; Greater than 40% identity with GRK7 and RK

Cross Reactivity:

Cross-reacts with GRK6

Protein Name(s):

Pan-GRK

Gene Name(s):

Grk5 Gprk5 Grk6 Gprk6

Antibody Registry ID:

Purified: AB_2232788
TC Supernatant: AB_10671656


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

70 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.