Human Mouse Rat
G protein-coupled receptor (GPCR) kinases (GRKs), are important regulators of GPCR function and mediate receptor desensitization, internalization, and signaling. Several non-GPCR proteins also serve as GRK substrates, and GRKs have been shown to have kinase-independent functions through regulation of receptors and effectors. These proteins are members of the G protein-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. GRKs phosphorylate activated forms of G protein-coupled receptors to regulate their signaling.
Fusion protein amino acids 381-590 (C-terminus) of mouse GRK5 (also known as G protein-coupled receptor kinase 5 and Gprk5, accession number Q8VEB1); Rat: 99% identity (208/210 amino acids identical); Human: 91% identity (193/210 amino acids identical); Greater than 55% identity with GRK6 and GRK4; Greater than 40% identity with GRK7 and RK
Cross-reacts with GRK6
Grk5 Gprk5 Grk6 Gprk6
TC Supernatant: AB_10671656
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.