Products: Primary Antibodies

Anti-Neuregulin-CRD (Cysteine-rich domain, Type III) (N126B/31)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-226	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-226	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 1-75 (membrane-associated N-terminus) of human Neuregulin-1 (also known as NRG1, Neu differentiation factor, NDF, Heregulin, HRG, HGL, Breast cancer cell differentiation factor p45, Acetylcholine receptor-inducing activity, ARIA, Glial growth factor, GGF, Sensory and motor-neuron-derived factor or SMDF, accession number Q96IB3)
Mouse: 85% identity (64/75 amino acids identical)
Rat: 82% identity (63/76 amino acids identical)

Cross Reactivity:

None Reported

Protein Name(s):

Neuregulin 1

Gene Name(s):

NRG1

Antibody Registry ID:

Purified: AB_10672367
TC Supernatant: AB_10672412

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

30 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Venkatesan K, Liu Y, Goldfarb M.. (2014), 'Fast-onset long-term open-state block of sodium channels by A-type FHFs mediates classical spike accommodation in hippocampal pyramidal neurons. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1271-14.2014.
Song F, Chiang P, Ravits J, Loeb JA.. (2014), 'Activation of microglial neuregulin1 signaling in the corticospinal tracts of ALS patients with upper motor neuron signs. .' Amyotroph Lateral Scler Frontotemporal Degener.. 10.3109/21678421.2013.853802.
Zhang K, Wong P, Zhang L, Jacobs B, Borden EC, Aster JC, Bedogni B.. (2012), 'A Notch1-neuregulin1 autocrine signaling loop contributes to melanoma growth. .' Oncogene. 10.1038/onc.2011.606.