Products: Primary Antibodies

Anti-Nav1.6 Na+ channel (K87A/10)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-026	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $325.00
Brand	Catalog # 73-026	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP

Immunogen:

Synthetic peptide 459-476 (intracellular interdomain loop I-II) of rat Nav1.6 (accession number NP_062139)

Human: 88% identity (16/18 amino acids identical)
Mouse: 100% identity (18/18 amino acids identical)

Cross Reactivity:

None Reported

Protein Name(s):

Sodium channel protein type 8 subunit alpha (Peripheral nerve protein type 4) (PN4) (Sodium channel 6) (NaCh6) (Sodium channel protein type VIII subunit alpha) (Voltage-gated sodium channel subunit alpha Nav1.6)

Gene Name(s):

Scn8a

Antibody Registry ID:

Purified: AB_2184197
TC Supernatant: AB_10673284

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

K87A/10 does not work for immunoblotting

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Benned-Jensen T, Christensen RK, Denti F, Perrier JF, Rasmussen HB, Olesen SP.. (2016), 'Live Imaging of Kv7.2/7.3 Cell Surface Dynamics at the Axon Initial Segment: High Steady-State Stability and Calpain-Dependent Excitotoxic Downregulation Revealed. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2631-15.2016.
Jones SL, Korobova F, Svitkina T.. (2014), 'Axon initial segment cytoskeleton comprises a multiprotein submembranous coat containing sparse actin filaments. .' The Journal of Biology. 10.1083/jcb.201401045.
O'Guin KN, Gruber RC, Raine CS, Guzik HM, Poulos BK, Shafit-Zagardo B.. (2014), 'Gas6 enhances axonal ensheathment by MBP+ membranous processes in human DRG/OL promyelinating co-cultures. .' ASN Neuro. 10.1042/AN20130022.
Cortes DF, Landis MK, Ottens AK.. (2012), 'High-capacity peptide-centric platform to decode the proteomic response to brain injury. .' Electrophoresis. 10.1002/elps.201200341.
Yap CC, Vakulenko M, Kruczek K, Motamedi B, Digilio L, Liu JS, Winckler B.. (2012), 'Doublecortin (DCX) mediates endocytosis of neurofascin independently of microtubule binding. .' Journal of Neuroscience. 10.1523/JNEUROSCI.5318-11.2012.
Galiano MR, Jha S, Ho TS, Zhang C, Ogawa Y, Chang KJ, Stankewich MC, Mohler PJ, Rasband MN.. (2012), 'A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly. .' Cell. 10.1016/j.cell.2012.03.039.
Dhaunchak AS, Becker C, Schulman H, De Faria O Jr, Rajasekharan S, Banwell B, Colman DR, Bar-Or A; Canadian Pediatric Demyelinating Disease Group.. (2012), 'Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis. .' Annals of Neurology. 10.1002/ana.22693.
Buffington SA, Sobotzik JM, Schultz C, Rasband MN.. (2012), 'IκBα is not required for axon initial segment assembly. .' Molecular and Cellular Neuroscience. 10.1016/j.mcn.2012.03.003.
Kaphzan H, Buffington SA, Jung JI, Rasband MN, Klann E.. (2012), 'Alterations in intrinsic membrane properties and the axon initial segment in a mouse model of Angelman syndrome. .' Journal of Neuroscience. 10.1523/JNEUROSCI.4162-11.2011.
Roza C, Castillejo S, Lopez-García JA.. (2011), 'Accumulation of Kv7.2 channels in putative ectopic transduction zones of mice nerve-end neuromas. .' Molecular Pain. 10.1186/1744-8069-7-58.
Shi Y, Sun W, McBride JJ, Cheng JX, Shi R.. (2011), 'Acrolein induces myelin damage in mammalian spinal cord. .' Journal of Neurochemistry. 10.1111/j.1471-4159.2011.07226.x.
Grubb MS, Burrone J.. (2010), 'Activity-dependent relocation of the axon initial segment fine-tunes neuronal excitability. .' Nature. 10.1038/nature09160.
Lorincz A, Nusser Z.. (2010), 'Molecular identity of dendritic voltage-gated sodium channels. .' Science. 10.1126/science.1187958.
Ogawa Y, Oses-Prieto J, Kim MY, Horresh I, Peles E, Burlingame AL, Trimmer JS, Meijer D, Rasband MN.. (2010), 'ADAM22, a Kv1 channel-interacting protein, recruits membrane-associated guanylate kinases to juxtaparanodes of myelinated axons. .' Journal of Neuroscience. 10.1523/JNEUROSCI.4661-09.2010.
Ogawa Y, Rasband MN.. (2009), 'Proteomic analysis of optic nerve lipid rafts reveals new paranodal proteins. .' Journal of Neuroscience Research. 10.1002/jnr.21984.
Lorincz A, Nusser Z.. (2008), 'Cell-type-dependent molecular composition of the axon initial segment..' Journal of Neuroscience. 10.1523/JNEUROSCI.4833-08.2008.
Hedstrom KL, Ogawa Y, Rasband MN.. (2008), 'AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity. .' The Journal of Biology. 10.1083/jcb.200806112.
Lonigro A, Devaux JJ.. (2009), 'Disruption of neurofascin and gliomedin at nodes of Ranvier precedes demyelination in experimental allergic neuritis. .' Brain. 10.1093/brain/awn281.
Micheva KD, Smith SJ.. (2007), 'Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits. .' Neuron. 0.1016/j.neuron.2007.06.014.
Van Wart A, Trimmer JS, Matthews G.. (2007), 'Polarized distribution of ion channels within microdomains of the axon initial segment. .' Journal of Comparative Neurology. 10.1002/cne.21173.