Products: Primary Antibodies

Anti-Nav1.6 Na+ channel Antibody (K87A/10)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-026	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $335.00
Brand	Catalog # 73-026	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $335.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP

Target Description:

Nav1.6 Na+ channel (sodium channel, voltage-gated, type 8, alpha subunit/ SCN8A) is a member of voltage-gated sodium ion channel subunit family. It is encoded by gene Scn8a in human.The channel switches between open and close conformation in response to the voltage difference accross the membrane. Nav1.6 Na+ channel is located at the nodes of Ranvier. It heavily participates in the regulation of nerve conduction velocity. It has been shown that Nav1.6 Na+ channel loss in roddents induces paralysis due to the failure of the neuromuscular junction (Wagnon et al., 2016).

Immunogen:

Synthetic peptide 459-476 (intracellular interdomain loop I-II) of rat Nav1.6 (accession number O88420); Human: 88% identity (16/18 amino acids identical); Mouse: 100% identity (18/18 amino acids identical)

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

Nav1.6 Na+ channel

Gene Name(s):

Scn8a

Antibody Registry ID:

Purified: AB_2184197
TC Supernatant: AB_10673284

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

K87A/10 does not work for immunoblotting

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.