Products: Primary Antibodies

Anti-GABA(B)R2 (N81/37)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-125	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-125	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 861-912 of rat GABABR2 (also known as gammaaminobutyric acid type B receptor subunit 2, GABA-B receptor 2, Gb2, Gprotein coupled receptor 51 or GPR51, accession number NP_113990)
Mouse: 100% identity (52/52 amino acids identical)
Human: 100% identity (52/52 amino acids identical)
<12% identity with GABABR1

Cross Reactivity:

No cross-reactivity against GABABR1

Protein Name(s):

Gamma-aminobutyric acid type B receptor subunit 2 (GABA-B receptor 2) (GABA-B-R2) (GABA-BR2) (GABABR2) (Gb2) (G-protein coupled receptor 51) (HG20)

Gene Name(s):

GABBR2 GPR51 GPRC3B

Antibody Registry ID:

Purified: AB_2278802
TC Supernatant: AB_10672297

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

105 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Terunuma M, Revilla-Sanchez R, Quadros IM, Deng Q, Deeb TZ, Lumb M, Sicinski P, Haydon PG, Pangalos MN, Moss SJ.. (2014), 'Postsynaptic GABAB receptor activity regulates excitatory neuronal architecture and spatial memory. .' Journal of Neuroscience. 10.1523/JNEUROSCI.3320-13.2013.
Marron Fernandez de Velasco E, Hearing M, Xia Z, Victoria NC, Luján R, Wickman K.. (2014), 'Sex differences in GABA(B)R-GIRK signaling in layer 5/6 pyramidal neurons of the mouse prelimbic cortex. .' Neuropharmacology. 10.1016/j.neuropharm.2015.03.029.
Hearing M, Kotecki L, Marron Fernandez de Velasco E, Fajardo-Serrano A, Chung HJ, Luján R, Wickman K.. (2013), 'Repeated cocaine weakens GABA(B)-Girk signaling in layer 5/6 pyramidal neurons in the prelimbic cortex. .' Neuron. 10.1016/j.neuron.2013.07.019.
Padgett CL, Lalive AL, Tan KR, Terunuma M, Munoz MB, Pangalos MN, Martínez-Hernández J, Watanabe M, Moss SJ, Luján R, Lüscher C, Slesinger PA.. (2013), 'Methamphetamine-evoked depression of GABA(B) receptor signaling in GABA neurons of the VTA. .' Neuron. 10.1016/j.neuron.2011.12.031.
Broussard DM, Titley HK, Antflick J, Hampson DR.. (2011), 'Motor learning in the VOR: the cerebellar component. .' Experimental Brain Research. 10.1007/s00221-011-2589-z.
Fernández-Alacid L, Aguado C, Ciruela F, Martín R, Colón J, Cabañero MJ, Gassmann M, Watanabe M, Shigemoto R, Wickman K, Bettler B, Sánchez-Prieto J, Luján R.. (2009), 'Subcellular compartment-specific molecular diversity of pre- and post-synaptic GABA-activated GIRK channels in Purkinje cells. .' Journal of Neurochemistry. 10.1111/j.1471-4159.2009.06229.x.