Products: Primary Antibodies

Anti-CALB1/calbindin Antibody (L109/57)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-448	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-448	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

AT ICC IHC WB

Target Description:

Calbindin (aka. calbindin D28k) is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is predominantly expressed in certain types of neurons, particularly in dendrites and perikarya of the cerebellum and is thought to buffer entry of calcium upon stimulation of glutamate receptors (Andressen et al., 1993). Calbindin has recently been shown to play a critical role in mitochondrial dysfunction and loss of synaptic proteins in vivo in an Alzheimer’s disease mouse model (Kook et al., 2014).

Immunogen:

Fusion protein amino acids 1-261 (full-length) of human CALB1 (also known as Calbindin D28, Vitamin D-dependent calcium-binding protein, D-28K, Spot 35 protein, CAB27 and PCD-29, accession number P05937)
Rat: 98% identity (257/261 amino acids identical)
Mouse: 98% identity (257/261 amino acids identical)
60% identity with CALB2/Calretinin

Protein Name(s):

Calbindin (Calbindin D28) (D-28K) (Vitamin D-dependent calcium-binding protein, avian-type)

Gene Name(s):

CALB1 CAB27

Antibody Registry ID:

Purified: AB_2686913
TC Supernatant: AB_2619740

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

30 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.