Anti-Vimentin Recombinant Chicken Chimeric mAb (BSA and Azide free) (LN6)

Name: Anti-Vimentin Recombinant Chicken Chimeric mAb (BSA and Azide free)
Brand: Aves Labs
SKU: 78-610-CF
Price: 522.0 USD
Availability: InStock

Our chicken Anti-Vimentin carrier-free recombinant monoclonal antibody is a chimeric antibody derived from clone LN6. It detects human Vimentin and is purified by affinity chromatography. Works in ELISA, WB, IHC.

Anti-Vimentin Recombinant Chicken Chimeric mAb (BSA and Azide free)

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Species Reactivity

Bovine, Canine, Human, Mouse, Pig, Rabbit, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 78-610-CF

Volume: 100 µL

Variant

Price:

$522.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Vimentin

Scientific Background

Vimentin is the major protein subunit of the 10 nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.6-1.0 mg/mL

Clonality

Recombinant

Clone

LN6

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

VIM

Molecular Weight

50 kDa

Excitation / Emission

Unconjugated

Immunogen

Cell nuclei preparation from adult human thymus.

Immunogen Species

Human

Species Reactivity

Bovine, Canine, Human, Mouse, Pig, Rabbit, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone LN6 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone LN6 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

PBS

Dilution Ranges

WB: 1:1000
IHC: 1:100-1:250
ICC: 1:100-1:500

Conjugate

Unconjugated, Carrier Free

Specificity

Does not cross-react with Arl13a (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by Western blot and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN; Vimentin

UniProt ID

P08670
UniProt Summary: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity). {ECO:0000250|UniProtKB:A0A8C0N8E3, ECO:0000250|UniProtKB:P20152, ECO:0000250|UniProtKB:P31000}.; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. {ECO:0000269|PubMed:21746880}.

NCBI Gene ID

7431

Shipping

Shipped on ice packs

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Anti-Vimentin Recombinant Chicken Chimeric mAb (BSA and Azide free) (LN6)

SKU: 78-610-CF

Product Details

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