Anti-Vimentin Recombinant Chicken Chimeric mAb FL594 Conjugate (LN6)
Our chicken Anti-Vimentin FL594 conjugate recombinant monoclonal antibody is a chimeric antibody derived from clone LN6. It detects human Vimentin and is purified by affinity chromatography. Works in IHC.
Anti-Vimentin Recombinant Chicken Chimeric mAb FL594 Conjugate
Vimentin is the major protein subunit of the 10 nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).
Purified by affinity chromatography
Lot dependent: provided at 0.3-0.5 mg/mL
Recombinant
LN6
IgY
FACS, ICC, IHC
Chicken
VIM
50 kDa
FL594 Ex: 594 nm, Em: 615 nm
Cell nuclei preparation from adult human thymus.
Human
Bovine, Canine, Human, Mouse, Pig, Rabbit, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone LN6 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone LN6 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
1X PBS, 0.05% Sodium Azide pH 7.4
IHC: 1:100-1:250
ICC: 1:100-1:500
594
Does not cross-react with Arl13a (based on KO validation results)
Each new lot of antibody is quality control tested by Western blot and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN; Vimentin
P08670
UniProt Summary: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity). {ECO:0000250|UniProtKB:A0A8C0N8E3, ECO:0000250|UniProtKB:P20152, ECO:0000250|UniProtKB:P31000}.; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. {ECO:0000269|PubMed:21746880}.
