Our Anti-Pan-GRK mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N145/20. It detects human, mouse, and rat Pan-GRK, and is purified by Protein A chromatography. It is great for use in ICC, WB.
G protein-coupled receptor (GPCR) kinases (GRKs), are important regulators of GPCR function and mediate receptor desensitization, internalization, and signaling. Several non-GPCR proteins also serve as GRK substrates, and GRKs have been shown to have kinase-independent functions through regulation of receptors and effectors. These proteins are members of the G protein-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. GRKs phosphorylate activated forms of G protein-coupled receptors to regulate their signaling.
Purified by Protein A chromatography
Grk5 Gprk5 Grk6 Gprk6
Fusion protein amino acids 381-590 (C-terminus) of mouse GRK5 (accession number Q8VEB1) produced recombinantly in E. Coli
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.107
Cross-reacts with GRK6
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.