Primary Antibodies

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Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N275/14 (left) and N86/8 (right).Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged ASIC1, ASIC2 or untagged Kv2.1 plasmid and probed with N271/44 (left) or N86/8 (right) TC supe.Adult rat brain immunohistochemistry.
Immunoblot against crude brain membrane preparations from adult rat (RBM) or wild-type (WT) or KA2 knockout (KO) mice probed with N279B/27 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Anis Contractor (Northwestern University.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS-1 cells transiently transfected with KCNQ1, KCNQ2, KCNQ3, KCNQ4, KCNQ5 or Kv2.1 plasmids and probed with pure N26A/23.
Adult rat brain membrane immunoblot.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N270/47 (left) and N86/8 (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin plasmid and probed with N269/73 (left) and N86/8 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N274/8 (left) and N86/8 (right).
Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe.
Adult rat brain immunohistochemistry.Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Clcn3 KO mouse brains probed with N258/5 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Fred Lamb (University of Iowa.
Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with untagged Cav1.2, Cav1.3 or Kv2.1 plasmids and probed with N263/31 (left) and L57/46 (right) TC supe.TIRF images (top row), super-resolution GSD localization maps (middle row) and binarized images (bottom row) of immunostained CaV1.2 (Cat. 75-257, 1:200) channels and β1ARs in representative fixed adult ventricular myocytes isolated from WT mice under control (A) or ISO-stimulated conditions (B). Merged two-channel image showing relative distributions of CaV1.2 and β1ARs is shown in the third column with the colocalized binarized image. Image from publication CC-BY-4.0. PMID:37438479
Immunoblot against adult rat brain membrane (RBM) or low- speed pellet (RBLSP) fractions denatured at 95oC for 5 min or 37oC for 15 min and probed with N253/32 (left) and K58/35 (right) TC supe.
Adult rat brain immunohistochemistry.Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N25/35 (left) or N52A/42 (right) TC supe.
Immunoblot against bacterially expressed Thioredoxin (TRX) and/or 6xHistidine (His) tagged fusion proteins.
Immunolabeling of mouse skeletal muscle staining NaV 1.4 (Cat. 75-424, 1:1000, red) and acetylcholine receptor (red). The frozen tissue section was fixed with 4% PFA for 10 minutes and mounted before staining. Image kindly provided by Chengjie Xie and Lauren Fish, Brown University.
Adult rat brain membrane immunoblot.Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged TARPGamma2/3/4/8 or untagged Kv2.1 plasmid and probed with N245/36 (left) and N86/8 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids and probed with N23B/6 TC supe (left panel) or a monoclonal lane loading control (right panel).
Adult rat sciatic nerve immunofluorescence staining: paranodes labeled with N244/5 (red) surround nodes of Ranvier labeled with Beta-IV spectrin (green) (image on right is cross-section of a similar image of left). Images courtesy of Matt Rasband (Baylor College of Medicine).Immunoblot versus adult rat brain membranes (RBM) and adult mouse brain membranes from wild-type (WT) and SynCAM4 knockout (KO) mice and probed with N244/5 (left) and K89/41 (right) TC supe. Samples courtesy of Matt Rasband, Baylor College of Medicine.
Cultured rat hippocampal neuron immunofluorescence: N23B/49 (Pan-Shank) = red, rabbit anti-PSD-95 = green, merge = yellow (bottom right). Image courtesy of Dr. Albert Hung, Picower Institute, MIT.Adult rat hippocampus immunohistochemistry.
NeuroMab Anti-Pan-Shank Antibody
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Immunoblot versus low-speed pellet samples from wild-type (WT) and Stargazer mutant (STG) mice and probed with N245/1 TC supe. Data courtesy of Susumu Tomita, Yale University.
Adult rat brain membrane (RBM) immunoblot.
Adult rat brain membrane (RBM) immunoblot.
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane immunoblot.
Tissue and transfected cell immunoblot: low speed pellet samples from adult rat brain (RBLSP) and extracts of COS cells transiently transfected with Myc-tagged PEX6 or untagged Kv2.1 plasmids and probed with N233/8 (left) and K89/34 (right).

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