Primary Antibodies

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Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged NSD1, NSD2, NSD3 or untagged Kv2.1 plasmids and probed with N312/10 (left), 12CA5 (middle) and K89/34 (right).Representative images of validation of NSD1 (Cat. 75-280, 1:750) protein levels by Western blot in JHU 011, Cal27, and FaDu cell lines with NSD1 knockdown after 72 h doxycycline induction. Image from publication, CC-BY-4.0, PMID: 38539515.
NeuroMab Anti-NSD1 Antibody
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Immunoblot versus extracts of heart tissue from WT and SUR2 KO mice probed with N323A/31 TC supe. Data courtesy of William Coetzee and Margaret Rice, New York University SOM.
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N309A/21 TC supe (left) and a rabbit polyclonal antibody control (right).
"Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N307/12 TC supe (left) and a rabbit polyclonal control (right). ."
Tissue and transfected cell immunoblot: extracts of rat eye and brain membrane (REM and RBM, respectively) and COS cells transiently transfected with Myc-tagged BBS5 or untagged Kv2.1 plasmid and probed with N304B/115 (left) and N52A/42 (right) TC supe.
Serial dilutions of BSA-conjugated target and non-target peptides dotted onto membrane and probed with N310/21 TC supe (left) and a rabbit polyclonal antibody control (right).
Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N299/39 TC supe (left) and a mouse loading control (right).
Serial dilutions of BSA-conjugated modified and unmodified nucleotides dotted onto membrane and probed with N297/59 TC supe (left) and a rabbit polyclonal control (right).
Immunofluorescence of the ventricular zone of E14 mouse brain (frozen section) labeled with Anti-Arl13b (red, Cat No. 75-287, 1:1000) and CEP152 (Green). The blue is DAPI staining of nuclear DNA. Image kindly provided by Koh-ichi Nagata, Nagoya University Graduate School of Medicine.Immunofluorescence staining of adult rat brain sections with N295B/66 (red, cilia) and N147/6 (green) TC supe.
NeuroMab Anti-Arl13b Antibody
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Adult rat brain immunohistochemistry.Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag-tagged BBS3/Arl6 or untagged Kv2.1 plasmid and probed with N302/10 (left) and K89/34 (right) TC supe.
Immunofluorescence staining of adult spinal cord sections from Brevican wild-type (WT) and knockout (KO) mice with N294A/10 (red) and Caspr rabbit polyclonal (green). Images courtesy of Kae-Jiun Chang and Matt Rasband (Baylor College of Medicine).
Immunoblot versus crude membranes from adult rat brain (RBM) and WT and TRIP8b KO mouse brains probed with N291C/22 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Dane Chetkovich (Northwestern University).Transfected cell immunoblot: extracts of COS cells transiently transfected with carboxy- terminally (C) and amino-terminally (N) GFP- tagged TRIP8b-1a-5, TRIP8b-1a-4 or untagged Kv2.1 plasmid and probed with N291C/22 (left) and N212/7 (right) TC supe.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot versus crude membrane preparations from adult rat brain (RBM) and Brevican wild-type (WT) and knockout (KO) mice and probed with N294A/6 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Matthew Rasband, Baylor College of Medicine.
Immunofluorescence against SUR1 WT (left) and null mutant (right) mouse striatum and stained with N289/16 (green) and commercial antibody against Tyrosine Hydroxylase (blue). Images courtesy of Paul Witkovsky, Jyoti Patel and Margaret Rice, New York University Medical Center.Adult rat hippocampus immunohistochemistry (with antigen retrieval via Na citrate pretreatment).
Immunohistochemistry with antigen retrieval via citrate pretreatment.Adult rat hippocampus immunohistochemistry under standard protocol.
Immunofluorescence staining of zebrafish sections at 5 days post- fertilization with N286/74 (green) and Synapsin 1/2 rabbit polyclonal (red). Left: optic tectum (TeO), inner plexiform layer (asterisk) and photoreceptor layer (arrow). Right: synapse-rich axonal projections in optic tectum. Image courtesy of Philip Washbourne (University of Oregon).Immunoblot versus homogenates from adult zebrafish muscle (Non- neuronal), brain (Neuronal) and purified brain postsynaptic densities (PSD) probed with N286/74 TC supe. Image courtesy of Philip Washbourne (University of Oregon).
Coronal sections of rat cerebellum, stained with human patient autoantibodies (E, red) and anti-vGlut1 (F, green labeling, Cat no 75-066, 1:300). Colocalization was not observed when mixing the anti-VGlut1 with the autoantibodies (merge G, red labeling + green labeling), In addition, autoantibody labeling was identified with Cy3 red labeling, while the antibodies were identified with Alexa488 green labeling. CC-BY-4.0 PMID: 37383228HEK293T transfectants expressing SLITRK1 or its mutants were co-cultured with hippocampal neurons. Two days after co-culture, induced pre-synapses were quantified through VGAT or VGLUT1 immunostaining and GFP. Image from publication CC-BY-4.0. PMID:36085162
NeuroMab Anti-VGlut1 Antibody
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Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged VGlut1, VGlut2, VGlut3 or Kv2.1 plasmid and probed with N29/29 (left) or N52A/42 (right) TC supe.Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N29/29 (left) and N52A/42 (right) TC supe.
NeuroMab Anti-VGlut2 Antibody
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Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP- tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N278/19 (left) and N86/8 (right).Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N277/7 (left) and N86/8 (right).
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N276A/15 (left) and N86/8 (right).

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