Primary Antibodies

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Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Shank1, Shank2, Shank3 or untagged Kv2.1 plasmid and probed with N367/51 (left) or N23B/49 (right) TC supe.
Immunoblot against adult rat brain membranes (RBM) and membranes from SVOP knockout (KO) and wild-type (WT) mice probed with N356/23 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Jia Yao and Sandra Bajjalieh (University of Washington.
Adult rat brain immunohistochemistry.Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged ATF4 or untagged ATF5 or Kv2.1 plasmid and probed with N360A/24 TC supe (left), Rabbit anti-ATF5 (middle) or K89/34 TC supe (right).
Transfected cell immunofluorescence: COS cells expressing Myc-tagged Flrt3. Red = Myc, Green = N353/13, Blue = Hoechst nuclear stain.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged NSD1, NSD2, NSD3 or untagged Kv2.1 plasmid and probed with N348/82 (left) and K89/34 (right) TC supe.
Immunofluorescence staining of adult hippocampus (sp = stratum pyrimidale, sl = stratum lucidum) from Stonin-2 wild-type (WT) and knockout (KO) mice with N346/9 (red) and K89/34 (green). Mouse brains courtesy of Fabian Feutlinske and Volker Haucke (Freie Universität Berlin).Immunoblot versus crude membranes from adult rat brain (RBM) and WT and Stonin-2 KO mouse brains probed with N346/9 (left), K89/34 (middle) and N52A/42 (right) TC supes. Mouse brains courtesy of Fabian Feutlinske and Volker Haucke (Freie Universität Berlin).
Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag- tagged REEP1, REEP2 or untagged Kv2.1 plasmid and probed with N345/51 (left) and K89/34 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Foxi2 or untagged Kv2.1 plasmid and probed with N349/96 TC supe (left), Rabbit anti-Flag (middle) or N52A/42 TC supe (right.
Immunofluorescence staining of a node of Ranvier in adult rat sciatic nerve with N343/26 (green) and bIV-spectrin rabbit polyclonal (red). Image courtesy of Kae-Jiun Chang and Matt Rasband (Baylor College of Medicine.
Adult rat brain immunohistochemistry.Transfected cell immunoblot: extracts of CV-1 fibroblast cells transfected with VGlut3 (+) or left untransfected (-) and probed with N34/34 TC supe. Image courtesy of Jeff Erickson, Louisiana State University Health Sciences Center.
Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N336B/83 (left) or N52A/42 (right) TC supe. Note that BAF53a is ubiquitously expressed in many different cell types, including COS cells.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged LRRK1, LRRK2 or untagged Kv2.1 plasmid and probed with N341/12 (left) or N241A/34 (right) TC supe.Immunoblot versus LRRK1-immunoprecipitated extracts from WT and LRRK1 KO mouse heart probed with N341/12 TC supe. Data courtesy of Paul Davies and Dario Alessi (University of Dundee, Scotland, UK.
Adult rat hippocampus immunohistochemistry.Immunoblot versus samples from adult rat brain (RBLSP) and WT and BAF53b KO mouse brains probed with N332B/15 (left) and N336B/83 (right) TC supes. Mouse samples courtesy of Ann Kuo and Gerald Crabtree (Stanford).
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged Alpha-2C and Alpha-2A adrenergic receptor or untagged Kv2.1 plasmid and probed with N330A/80 TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged NR2A, NR2B or untagged Kv2.1 plasmid and probed with N327A/38 (left) and N86/38 (right) TC supe.
Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged Alpha-2C and Alpha-2A adrenergic receptor or untagged Kv2.1 plasmid and probed with N330A/51 TC supe.
Adult rat hippocampus immunohistochemstry.Adult rat brain membrane immunoblot.
Adult rat brain membrane immunoblot.Adult rat brain immunohistochemistry.
Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with V5/His-tagged THAP1 or untagged Kv2.1 plasmid and probed with N325B/65 TC supe (left) and mouse anti-V5 (right).
Tissue and transfected cell immunoblot: extracts of rat brain membrane (RBM) and COS cells transiently transfected with Flag-tagged Kirrel3-short, Kirrel3-long, untagged Kirrel2, Kirrel1 or Kv2.1 plasmid and probed with N321C/49 (left) and N52A/42 (right) TC supe.
Adult rat hippocampus immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Kirrel3-short, Kirrel3-long or untagged Kv2.1 plasmids and probed with N320/48 (left) and Mouse anti-Flag (right).
Immunoblot versus extracts of heart tissue from WT and SUR2 KO mice probed with N319A/14 TC supe. Data courtesy of William Coetzee and Margaret Rice, New York University SOM.

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