Anti-VGlut2 Recombinant Chicken Chimeric mAb (N29/29)

Name: Anti-VGlut2 Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-067
Price: 481.0 USD
Availability: InStock

Our chicken Anti-VGlut2 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N29/29. It detects chicken, electric ray, human, mouse, and rat VGlut2 and is purified by affinity chromatography. Works in WB, IHC, ICC, ELISA.

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of VGlut2 with anti-VGlut2 recombinant chicken monoclonal antibody (green) (Cat. 78-067) at 1:100 dilution. The sections were mounted with FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-VGlut2 specifically stains the mossy fiber terminals in the granular layer and climbing climbing fiber terminals in the molecular layer of the cerebellum. Magnification 100X.

$Western blotting of rat brain membrane fraction with Aves Labs anti-Vglut2 recombinant chicken m (Cat. 78-067) antibody and detected with anti-chicken-HRP. Aves Labs recombinant chicken anti-VGlut2 mAb recognizes endogenous Vglut2 in the rat brain membrane fraction at ~60 kDa.$

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of VGlut2 with anti-VGlut2 recombinant chicken monoclonal antibody (green) (Cat. 78-067) at 1:100 dilution. The sections were mounted with FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-VGlut2 specifically stains the mossy fiber terminals in the granular layer and climbing climbing fiber terminals in the molecular layer of the cerebellum. Magnification 100X.

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Species Reactivity

Chicken, Electric Ray, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-067

Volume: 100 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VGlut2

Scientific Background

Vesicular glutamate transporter 2 (VGlut2) is encoded by the gene Slc17a6 and belongs to the vesicular glutamate transporter family. Vglut2 is expressed in the thalamus, midbrain, brainstem, and spinal cord at synaptic vesicles. It functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut2 has been implicate in include Gnathodiaphyseal Dysplasia and tendinosis.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N29/29

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Slc17a6 Dnpi Vglut2

Molecular Weight

60 kDa

Immunogen

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2 (accession number Q9JI12) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Chicken, Electric Ray, Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N29/29 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N29/29 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:400
IHC: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Does not cross-react with VGlut1 or VGlut3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Vesicular glutamate transporter 2 (VGluT2) (Differentiation-associated BNPI) (Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter) (Solute carrier family 17 member 6)

UniProt ID

Q9JI12

Shipping

Shipped on ice packs

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Anti-VGlut2 Recombinant Chicken Chimeric mAb (N29/29)

SKU: 78-067

Product Details

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