Anti-VGlut1 Recombinant Chicken Chimeric mAb (N28/9)

Name: Anti-VGlut1 Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-066
Price: 481.0 USD
Availability: InStock

Our chicken Anti-VGlut1 recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N28/9. It detects fish, human, mouse, and rat VGlut1 and is purified by affinity chromatography. Works in WB, IHC, ICC, Flow, EM, ELISA.

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of VGlut1. The sections were stained with anti-VGlut1 recombinant chicken monoclonal antibody (green) (Cat. 78-066) at 1:100 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-VGlut1 specifically stains the axonal synapses in the granular layer of the cerebellum. Magnification 100X.

$Western blotting of rat brain membrane fraction with Aves Labs anti-Vglut1 recombinant chicken Mab (Cat. 78-066) antibody at various concentrations and detected with anti-chicken-HRP. Aves Labs Recombinant Chicken anti-VGlut1 Mab recognizes endogenous Vglut1 in the rat brain membrane fraction at ~52 kDa.$

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of VGlut1. The sections were stained with anti-VGlut1 recombinant chicken monoclonal antibody (green) (Cat. 78-066) at 1:100 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-VGlut1 specifically stains the axonal synapses in the granular layer of the cerebellum. Magnification 100X.

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Species Reactivity

Fish, Human, Mouse, Rat

Applications

ELISA, EM, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-066

Volume: 100 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VGlut1

Scientific Background

Vesicular glutamate transporter 1 (Vglut1) is encoded by the gene Slc17a7 and belongs to the vesicular glutamate transporter family. Vglut1 is expressed in glutamatergic neurons in brain regions including the cerebellum, cerebral cortex and hippocampus at synaptic vesicles. Isoform 2 is expressed in retinal. Vglut1 functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut1 has been implicated in several neurological and psychiatric diseases including Alzheimer’s disease, Parkinson’s disease, epilepsy and schizophrenia.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N28/9

Isotype

IgY

Applications

ELISA, EM, ICC, IHC, WB

Host Species

Chicken

Gene Name

Slc17a7 Bnpi Vglut1

Molecular Weight

52 kDa

Immunogen

Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession number Q62634) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Fish, Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N28/9 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N28/9 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

Does not cross-react with VGlut2 or VGlut3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Vesicular glutamate transporter 1 (VGluT1) (Brain-specific Na(+)-dependent inorganic phosphate cotransporter) (Solute carrier family 17 member 7)

UniProt ID

Q62634

Shipping

Shipped on ice packs

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Anti-VGlut1 Recombinant Chicken Chimeric mAb (N28/9)

SKU: 78-066

Product Details

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