Anti-VGlut1 Recombinant Chicken Chimeric mAb Biotin Conjugate (N28/9)

Name: Anti-VGlut1 Recombinant Chicken Chimeric mAb Biotin Conjugate
Brand: Aves Labs
SKU: 78-066-BTN
Price: 562.0 USD
Availability: InStock

Our chicken VGlut1 biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone N28/9. It detects fish, human, mouse, and rat VGlut1 and is purified by affinity chromatography. Works in ELISA, IHC, ICC, Flow, WB.

Anti-VGlut1 Recombinant Chicken Chimeric mAb Biotin Conjugate

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Species Reactivity

Fish, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-066-BTN

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

VGlut1

Scientific Background

Vesicular glutamate transporter 1 (Vglut1) is encoded by the gene Slc17a7 and belongs to the vesicular glutamate transporter family. Vglut1 is expressed in glutamatergic neurons in brain regions including the cerebellum, cerebral cortex and hippocampus at synaptic vesicles. Isoform 2 is expressed in retinal. Vglut1 functions to uptake glutamate into synaptic vesicles in excitatory neural cells. Altered expression of Vglut1 has been implicated in several neurological and psychiatric diseases including Alzheimer’s disease, Parkinson’s disease, epilepsy and schizophrenia.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

N28/9

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

Slc17a7 Bnpi Vglut1

Molecular Weight

52 kDa

Immunogen

Fusion Protein Amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession number Q62634) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Fish, Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N28/9 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N28/9 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

IHC: 1:100-1:200
ICC: 1:100-1:200

Conjugate

Biotin

Specificity

Does not cross-react with VGlut2 or VGlut3

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

BNPI; Vesicular glutamate transporter 1; VGluT1; Brain-specific Na(+-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 7

UniProt ID

Q62634
UniProt Summary: Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate. At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane. In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification. Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake. The vesicular K(+)/H(+) antiport activity is electroneutral. At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation. The symporter activity is driven by an inside negative membrane potential and is electrogenic. Is necessary for synaptic signaling of visual-evoked responses from photoreceptors.

NCBI Gene ID

116638

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Anti-VGlut1 Recombinant Chicken Chimeric mAb Biotin Conjugate (N28/9)

SKU: 78-066-BTN

Product Details

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