Anti-Pink1 PTEN Induced Putative Kinase 1 Recombinant Chicken Chimeric mAb Biotin Conjugate (N4/15)

Name: Anti-Pink1 PTEN Induced Putative Kinase 1 Recombinant Chicken Chimeric mAb Biotin Conjugate
Brand: Aves Labs
SKU: 78-060-BTN
Price: 562.0 USD
Availability: InStock

Our chicken Pink1 PTEN induced putative kinase 1 biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone N4/15. It detects human, mouse, and rat Pink1 PTEN induced putative kinase 1 and is purified by affinity chromatography. Works in ELISA, IHC, ICC, WB.

Anti-Pink1 PTEN Induced Putative Kinase 1 Recombinant Chicken Chimeric mAb Biotin Conjugate

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Carrier-Free

Go to Full Product Details

Data Sheet

SKU: 78-060-BTN

Volume: 200 µL

Variant

Price:

$562.00

Quantity:

Ships: 1-5 business days

Product Details

Target

Pink1 PTEN induced putative kinase 1

Scientific Background

PTEN Induced Kinase 1 is encoded by the gene PINK1. PINK1 is a member of the protein kinase superfamily, Ser/Thr protein kinase family. PINK1 is a serine/threonine protein kinase that protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. PINK1 is highly expressed in the heart, skeletal muscle, and testis. Diseases associated with PINK1 include Parkinson Disease 6, Autosomal Recessive Early-Onset and Parkinson Disease 2, Autosomal Recessive Juvenile.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.3-0.5 mg/mL

Clonality

Recombinant

Clone

N4/15

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Gene Name

PINK1

Molecular Weight

63 kDa

Immunogen

Fusion Protein Amino acids 112-496 of human Pink1 (accession number NP_115785) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N4/15 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N4/15 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

IHC: 1:200-1:1000
ICC: 1:200-1:1000

Conjugate

Biotin

Specificity

No cross-reactivity reported

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Synonyms

BRPK; Serine/threonine-protein kinase PINK1; PTEN-induced putative kinase protein 1; EC 2.7.11.1; protein kinase BRPK; mitochondrial

UniProt ID

Q9BXM7
UniProt Summary: Serine/threonine-protein kinase which acts as a sensor of mitochondrial damage and protects against mitochondrial dysfunction during cellular stress. It phosphorylates mitochondrial proteins to coordinate mitochondrial quality control mechanisms that remove and replace dysfunctional mitochondrial components. In healthy mitochondria, PINK1 is translocated across the mitochondrial outer membrane (MOM) via the translocase of the outer membrane (TOM) complex, and inserted into the mitochondrial inner membrane (MIM) via the translocase of the inner membrane (TIM23) complex where it is cleaved and released into the cytosol. Depending on the severity of mitochondrial damage, activity ranges from preventing apoptosis and stimulating mitochondrial biogenesis to eliminating severely damaged mitochondria via PINK1-PRKN-dependent mitophagy. When cellular stress results in irreversible mitochondrial damage, PINK1 accumulates at the outer mitochondrial membrane (OMM) where it phosphorylates pre-existing polyubiquitin chains at 'Ser-65', recruits PRKN from the cytosol to the OMM and activates PRKN by phosphorylation at 'Ser-65'; activated PRKN then ubiquitinates VDAC1 and other OMM proteins to initiate mitophagy. The PINK1-PRKN pathway also promotes fission of damaged mitochondria through phosphorylation and PRKN-dependent degradation of mitochondrial proteins involved in fission such as MFN2. This prevents the refusion of unhealthy mitochondria with the mitochondrial network or initiates mitochondrial fragmentation facilitating their later engulfment by autophagosomes. Also promotes mitochondrial fission independently of PRKN and ATG7-mediated mitophagy, via the phosphorylation and activation of DNM1L. Regulates motility of damaged mitochondria by promoting the ubiquitination and subsequent degradation of MIRO1 and MIRO2; in motor neurons, this likely inhibits mitochondrial intracellular anterograde transport along the axons which probably increases the chance of the mitochondria undergoing mitophagy in the soma. Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10. Phosphorylates LETM1, positively regulating its mitochondrial calcium transport activity.

NCBI Gene ID

65018

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-Pink1 PTEN Induced Putative Kinase 1 Recombinant Chicken Chimeric mAb Biotin Conjugate (N4/15)

SKU: 78-060-BTN

Product Details

Bulk Order Anti-Pink1 PTEN Induced Putative Kinase 1 Recombinant Chicken Chimeric mAb Biotin Conjugate

Related Products

Recently Viewed