Anti-Shank3 Antibody (N69/46)

Name: Anti-Shank3 Antibody
Brand: NeuroMab
SKU: 75-109
Price: 337.0 USD
Availability: InStock

Our mouse Shank3 monoclonal antibody from NeuroMab is produced in-house from clone N69/46. It detects human, mouse, and rat Shank3 and is purified by Protein A chromatography. Works in WB, IHC, ICC, IP, ELISA.

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2, Shank3 or Kv2.1 plasmids and probed with N69/46 TC supe (left panel) or a monoclonal lane loading control (right panel).

Adult rat hippocampus immunohistochemistry.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 75-109

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Shank3

Scientific Background

SH3 and multiple ankyrin repeat domains protein 3, or shank 3 (also known as proline-rich synapse-associated protein 2 (ProSAP2)) is a member of the shank family. Shank 3 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Shank 3 is found in many tissues including heart and spleen, but is most abundantly expressed in brain at the post synaptic density. Shank 3 acts as a scaffold protein anchoring and connecting membrane bound receptors, ion channels and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 3 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 3 gene have been associated with neuropsychiatric diseases, including autism spectrm disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N69/46

Isotype

IgG2b

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Shank3 Prosap2

Molecular Weight

190 kDa

Immunogen

Synthetic peptide amino acids 840-857 (PEKLPGSLRKGIPRTKSV) of rat Shank3 (accession number Q9JLU4)

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2187730

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.37

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity against Shank1 or Shank2

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

UniProt ID

Q9JLU4

Shipping

Shipped on ice packs

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Anti-Shank3 Antibody (N69/46)

SKU: 75-109

Product Details

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