Anti-PSD-95 Recombinant Chicken Chimeric mAb Biotin Conjugate (K28/43)
Our chicken PSD 95 biotin conjugate recombinant antibody from Aves Labs is produced in-house from clone K28/43. It detects chicken, human, mouse, non-human primate, and rat PSD 95 and is purified by affinity chromatography. KO validated. Works in ELISA, ICC, IHC, WB, IP.
Anti-PSD-95 Recombinant Chicken Chimeric mAb Biotin Conjugate
PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003). Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy
Purified by affinity chromatography
Lot dependent: provided at 0.3-0.5 mg/mL
Recombinant
K28/43
IgY
ELISA, ICC, IHC, IP, WB
Chicken
DLG4 PSD95
95-110 kDa (varies with cell background due to phosphorylation)
Fusion Protein Amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli
Human
Chicken, Human, Mouse, Non-Human Primate, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone K28/43 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone K28/43 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.
1X PBS, 0.05% Sodium Azide pH 7.4
IHC: 1:500
ICC: 1:500
Biotin
No cross-reactivity against recombinant Chapsyn-110, SAP97 or SAP102
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Discs large homolog 4 antibody; Disks large homolog 4; Disks large homolog 4 antibody; Postsynaptic density protein 95; DLG 4 antibody; PSD-95; Dlg4 antibody; Synapse-associated protein 90; DLG4_HUMAN antibody; SAP-90; FLJ97752 antibody; SAP90; FLJ98574 antibody; Human post synaptic density protein 95 antibody; Post synaptic density protein 95 antibody; Postsynaptic density protein 95 antibody; PSD 95 antibody; PSD-95 antibody; PSD95 antibody; SAP 90 antibody; SAP-90 antibody; SAP90 antibody; Synapse associated protein 90 antibody; Synapse-associated protein 90 antibody; Tax interaction protein 15 antibody
P78352
UniProt Summary: Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression. Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins.
1742
Shipped on ice packs
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