Anti-Neurofilament heavy polypeptide, phosphorylated, (pNF-H) Antibody

Our Anti-Neurofilament heavy polypeptide, phosphorylated (pNF-H) rabbit polyclonal primary antibody detects bovine, horse, human, mouse, other mammals (predicted), pig, and rat Neurofilament heavy polypeptide, phosphorylated (pNF-H), and is whole serum. It is validated for use in IF, ICC, IHC, WB.

Image of a rat spinal cord section by Immunofluorescence and Immunohistochemistry. The section was stained with R-1388-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Rabbit pAb, (green) and co-stained with product M-1407-100, Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), Mouse mAb, (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
Image of a mouse hippocampus section by Immunofluorescence and Immunohistochemistry. The section was stained with R-1388-50, Neurofilament heavy polypeptide, phosphorylated, (pNF-H), Rabbit pAb, (red, 1:2,000) and co-stained with product M-1384-50, Myelin basic protein (MBP), Clone 7G7, Mouse mAb,(green, 1:5,000). The blue is DAPI staining of nuclear DNA. Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.
Analysis by western blot of pNF-H expression in tissue lysates with R-1388-50, Neurofilament heavy polypeptide, phosphorylated, (pNF-H), Rabbit pAb (green 1:10,000) with a strong band at spprox 220 kDa corresponding to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected with this antibody. Lane 1: protein standard; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord lysate.
Image of a rat spinal cord section by Immunofluorescence and Immunohistochemistry. The section was stained with R-1388-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Rabbit pAb, (green) and co-stained with product M-1407-100, Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), Mouse mAb, (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
Image of a rat spinal cord section by Immunofluorescence and Immunohistochemistry. The section was stained with R-1388-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Rabbit pAb, (green) and co-stained with product M-1407-100, Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), Mouse mAb, (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
Click on image to zoom

Bovine, Horse, Human, Mouse, Pig, Rat
ICC, IF, IHC, WB
Rabbit

Go to Full Product Details

Data Sheet

SKU: R-1388-50

Volume/Size: 50 µL
Price:
$327.00

Bulk Order Anti-Neurofilament heavy polypeptide, phosphorylated, (pNF-H) Antibody

Related Products

Recently Viewed