Anti-Autophagy protein 5 (ATG5) Antibody

Our Anti-Autophagy protein 5 (ATG5) rabbit polyclonal primary antibody detects human Autophagy protein 5 (ATG5), and is IgG. It is validated for use in IHC-Frozen, WB.

Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 15% Horse serum, Primary antibody: Rabbit Polyclonal antibody to ATG5 (cat # R-138-500, 2 ug per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Donkey anti-rabbit PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Normal Rabbit Polyclonal IgG was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of ATG5 expressed in human neuroblastoma SH-SY5Y cell line using cat # R-138-500.
Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 15% Horse serum, Primary antibody: Rabbit Polyclonal antibody to ATG5 (cat # R-138-500, 2 ug per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Donkey anti-rabbit PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Normal Rabbit Polyclonal IgG was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of ATG5 expressed in human neuroblastoma SH-SY5Y cell line using cat # R-138-500.
Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 15% Horse serum, Primary antibody: Rabbit Polyclonal antibody to ATG5 (cat # R-138-500, 2 ug per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Donkey anti-rabbit PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Normal Rabbit Polyclonal IgG was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of ATG5 expressed in human neuroblastoma SH-SY5Y cell line using cat # R-138-500.
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Human
IHC, WB
Rabbit

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Data Sheet

SKU: R-138-500

Volume/Size: 500 µg
Price:
$327.00

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