Anti-Neurofilament Heavy, phosphorylated and non-phosphorylated (pNF-H) Antibody

Our Anti-Neurofilament heavy polypeptide, phosphorylated and non-phosphorylated (pNF-H) rabbit polyclonal primary antibody detects human, other mammals (predicted), and rat Neurofilament heavy polypeptide, phosphorylated and non-phosphorylated (pNF-H), and is whole serum. It is validated for use in IF, ICC, WB.

Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. The cultures were stained with R-1389-50, Neurofilament heavy polypeptide, phosphorylated and non-phosphorylated (pNF-H), Rabbit pAb (green), and co-stained with product C-1373-50, Glial Fibrillary Acidic Protein (GFAP), Chicken pAb, (red). The neurofilament NF-H antibody binds to phosphorylated axonal forms of NF-H and non-phosphorylated dendritic and perikaryal forms.
Image of membrane TrkC expression (green) in human HeLa cells by Immunofluorescence and Immunocytochemistry. Method: Fixed (4% formaldehyde) and blocked (10% normal horse serum) HeLa cells were incubated with product M-1837-100, Tyrosine Kinase Receptor C (TrkC), Clone BS337, Mouse mAb, (green, 2 µg/mL) for 1 hour. Cells were then permeabilized, blocked (10% horse serum, 0.1% Triton X-100) and incubated with product R-1389-50 (red, 1:500) for 1 hour. Primary antibody binding was visualized with secondary donkey anti-mouse-CF488A and donkey anti-rabbit-CF568 antibodies (2 µg/mL, 1 hour incubation). Cell nuclei were stained with DAPI (blue). Negative control staining was performed under identical conditions with isotype control mouse antibody and rabbit whole serum from an non-immunized animal. Confocal imaging settings were the same for antibody and control staining. Magnification: 60x. Specific staining is observed for TrkC in the cell membrane, while the NF-H antibody reveals intracellular filaments.
Image of of membrane TrkC expression (green) in human HeLa cells by 3D confocal imaging . TrkC-IR is concentrated in the elongated protrusion away from the cell body, while intracellular NF-H expression (red) is observed throughout the cell. The blue is DAPI staining of nuclear DNA
Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. The cultures were stained with R-1389-50, Neurofilament heavy polypeptide, phosphorylated and non-phosphorylated (pNF-H), Rabbit pAb (green), and co-stained with product C-1373-50, Glial Fibrillary Acidic Protein (GFAP), Chicken pAb, (red). The neurofilament NF-H antibody binds to phosphorylated axonal forms of NF-H and non-phosphorylated dendritic and perikaryal forms.
Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. The cultures were stained with R-1389-50, Neurofilament heavy polypeptide, phosphorylated and non-phosphorylated (pNF-H), Rabbit pAb (green), and co-stained with product C-1373-50, Glial Fibrillary Acidic Protein (GFAP), Chicken pAb, (red). The neurofilament NF-H antibody binds to phosphorylated axonal forms of NF-H and non-phosphorylated dendritic and perikaryal forms.
Click on image to zoom

Human, Rat
ICC, IF, WB
Rabbit

Go to Full Product Details

Data Sheet

SKU: R-1389-50

Volume/Size: 50 µL
Price:
$327.00

Bulk Order Anti-Neurofilament Heavy, phosphorylated and non-phosphorylated (pNF-H) Antibody

Related Products

Recently Viewed