Anti-Laminin-111 Antibody
Our Anti-Laminin-111 rabbit polyclonal primary antibody detects mouse and rat Laminin-111, and is affinity purified. It is validated for use in IHC-Frozen, WB.
![Left: Analysis of rat brain stem section stained with rabbit anti-laminin (red, 1:1,000), and chicken anti-myelin basic protein (MBP, C-1383-50, green, 1:5,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained.The laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons. Right: Western blot analysis of laminin expression (green, 1:5,000). [2] rat brain, [3] rat spinal cord, and [4] cow spinal cord. The strong band above the 250 kDa mark corresponds to full-length laminin protein. Smaller proteolytic fragments of laminin are also detected with this antibody.](http://www.antibodiesinc.com/cdn/shop/files/r-1808-100-ihc-wb-2_120x120.jpg?v=1759274850)
Left: Staining of laminin in mouse section of cortex by Immunohistochemistry. Rabbit antibody to Laminin (red) reveals strong staining in the basement membranes of blood vessels. Blue: DAPI nuclear stain. Right: Western blot analysis of rat heart cell lysate (Lane 1) and 0.2 ug of purified laminin-111 protein from mouse EHS sarcoma (Lane 2). This antibody recognizes 3 laminin isotypes: alpha-1 (440 kDa), beta-1 (220 kD) and gamma-1 (220 kDa). Also recognized is a laminin-binding protein at 120 kDa in both rat heart lysate and purified laminin protein. Since this protein always co-expresses with laminin this crossreactivity is irrelevant.
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