Anti-Methyl-CpG- binding protein 2 (MeCP2) Antibody
Our Anti-Methyl-CpG- binding protein 2 (MeCP2) rabbit polyclonal primary antibody detects human, mouse, and rat Methyl-CpG- binding protein 2 (MeCP2), and is affinity purified. It is validated for use in IHC-Frozen, WB.
![Left: Analysis of rat cerebellum with rabbit anti-MeCP2 (red, 1:5,000) and chicken anti-calbindin (C-1798-50, green, 1:2,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The MeCP2 antibody selectively stains nuclei of neuronal cells. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer. Right: Western blot analysis of tissue and cell lysates using rabbit anti-MeCP2 (1:20,000). [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight of ~54 kDa.](http://www.antibodiesinc.com/cdn/shop/files/r-1810-100-ihc-wb-2_120x120.jpg?v=1759274855)
Left: Staining of mouse brain section with rabbit antibody to MeCP2 (green) by Immunohistochemistry. MeCP2 is mainly associated with methylated DNA in nucleus of neuronal cells. Blue: DAPI nuclear stain. IHC method: Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed overnight and cut to 45 uM. Right: Western blot analysis of MeCP2 expression in nuclear extracts from mouse brain. This antibody recognizes a strong and clear band at 74 kDa corresponding to MeCP2. The molecular weight of MeCP2 is 52 KDa, however, on SDS-PAGE this protein migrates slower due to its unusual high positive charge density.
Click on image to zoom
