Anti-Ki-67 Antibody

Our Anti-Ki-67 rabbit polyclonal primary antibody detects human Ki-67, and is whole serum. It is validated for use in ICC, WB.

Left: Immunofluorescence analysis of HeLa cells costained with rabbit antibody to Ki-67 (1:5,000, red) and a mouse antibody to beta-tubulin. Ki-67 is predominantly localized in nucleoli of cells in interphase, while in mitotic cells Ki-67 is associated with condensed chromosomes. Ki-67 is not expressed in cells in quiescent G0 state. Blue: DAPI staining of nuclear DNA. Right: Western blots analysis of equal amounts of HeLa (Lane 2) and HEK293 (Lane 3) cell lysates using rabbit antibody to Ki-67 (1:10,000). Double bands above 250 kDa correspond to two major Ki-67 isoforms of molecular weight ~345 and 395 kDa. Smaller fragments of these isoforms are also detected on the blot.
Analysis of Ki67 expression in cultured HeLa cells with rabbit anti-Ki67 antibody (red, 1:5,000), and mouse anti-fibrillarin (M-1372-250, green, 1:2,000) by Immunocytochemistry. Blue: DAPI nuclear stain. Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative, but fibrillarin-positive.
Left: Immunofluorescence analysis of HeLa cells costained with rabbit antibody to Ki-67 (1:5,000, red) and a mouse antibody to beta-tubulin. Ki-67 is predominantly localized in nucleoli of cells in interphase, while in mitotic cells Ki-67 is associated with condensed chromosomes. Ki-67 is not expressed in cells in quiescent G0 state. Blue: DAPI staining of nuclear DNA. Right: Western blots analysis of equal amounts of HeLa (Lane 2) and HEK293 (Lane 3) cell lysates using rabbit antibody to Ki-67 (1:10,000). Double bands above 250 kDa correspond to two major Ki-67 isoforms of molecular weight ~345 and 395 kDa. Smaller fragments of these isoforms are also detected on the blot.
Left: Immunofluorescence analysis of HeLa cells costained with rabbit antibody to Ki-67 (1:5,000, red) and a mouse antibody to beta-tubulin. Ki-67 is predominantly localized in nucleoli of cells in interphase, while in mitotic cells Ki-67 is associated with condensed chromosomes. Ki-67 is not expressed in cells in quiescent G0 state. Blue: DAPI staining of nuclear DNA. Right: Western blots analysis of equal amounts of HeLa (Lane 2) and HEK293 (Lane 3) cell lysates using rabbit antibody to Ki-67 (1:10,000). Double bands above 250 kDa correspond to two major Ki-67 isoforms of molecular weight ~345 and 395 kDa. Smaller fragments of these isoforms are also detected on the blot.
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Human
ICC, WB
Rabbit

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Data Sheet

SKU: R-1806-100

Volume/Size: 100 µL
Price:
$327.00

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