Anti-GABA-A-R-Beta2/3 Antibody (BSA and Azide free) (62-3G1)

Name: Anti-GABA-A-R-Beta2/3 Antibody (BSA and Azide free)
Brand: NeuroMab
SKU: 75-363-CF
Price: 486.0 USD
Availability: InStock

Our mouse GABA-A-R-Beta2/3 carrier-free monoclonal antibody from NeuroMab is produced in-house from clone 62-3G1. It detects bovine, chicken, human, mouse, and rat GABA-A-R-Beta2/3 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Anti-GABA-A-R-Beta2/3 Antibody (BSA and Azide free)

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Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

Unconjugated

490

550

594

650

Biotin

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Data Sheet

SKU: 75-363-CF

Volume: 100 µL

Variant

Price:

$486.00

Quantity:

Ships: 1-5 business days

Product Details

Target

GABA-A-R-Beta2/3

Scientific Background

GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR-beta2/3 a is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR beta2/3 has been found to be expressed in brain at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane.

Format

Purified by Protein A chromatography

Concentration

Lot dependent: provided at 0.6-1.0 mg/mL

Clonality

Monoclonal

Clone

62-3G1

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

GABRB3

Molecular Weight

55 kDa

Immunogen

Fraction of bovine brain GABA-A receptor affinity purified against immobilized benzodiazepine Ro7-1986/1 (1988 de Blas et al J Neurosci PMID 2828565, 1988 Vitorica et al J Neurosci PMID 2828566). Target initially identified as a GABA-A receptor by: 1). rat brain immunocytochemistry staining pattern via light microscopy identical to that seen via ligand autoradiography with 3H-muscimol; 2). immunoprecipitation of 3H-muscimol-binding activity of the purified receptor complex; 3). recognition of ~57 kDa protein in immunoblots against crude rat brain membrane fractions Binding epitope identified to be within amino acids 26-40 (QSVNDPGNMSFVKET, extracellular N-terminus, 1992 Ewert et al Brain Res, PMID 1377081) of human GABA-A-R-Beta3 (also known as Gamma-aminobutyric acid receptor subunit beta-3, accession number P28472)

Immunogen Species

Bovine

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and buffer exchanged into 1X PBS. Purified mAbs are >90% specific antibody.

Buffer

PBS

Conjugate

Unconjugated

Specificity

Cross-reacts with GABA-A-R-Beta2 Does not cross-react with GABA-A-RBeta1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from date of receipt

Synonyms

Gamma-aminobutyric acid receptor subunit beta-3 (GABA(A) receptor subunit beta-3)

UniProt ID

P28472

Shipping

Shipped on ice packs

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Anti-GABA-A-R-Beta2/3 Antibody (BSA and Azide free) (62-3G1)

SKU: 75-363-CF

Product Details

Bulk Order Anti-GABA-A-R-Beta2/3 Antibody (BSA and Azide free)

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