Anti-GABA-A-R-Beta2/3 Antibody (62-3G1)

Name: Anti-GABA-A-R-Beta2/3 Antibody
Brand: NeuroMab
SKU: 75-363
Price: 308.0 USD
Availability: InStock

from NeuroMab Datasheet

1 Citation

Available Conjugates

490

550

594

650

This antibody recognizes an epitope found in both GABA AR beta 2 and 3.

Product Type

Primary Antibody

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Clone

62-3G1

Applications

EM, ICC, IHC, IP, WB

Host Species

Mouse

Format

Purified by Protein A chromatography

Gene Name

GABRB3

Molecular Weight

55 kDa

Go to Full Product Details

Adult rat whole brain immunohistochemistry

Adult rat brain membrane immunoblot

Volume: 100 µL

Variant

Price:

$308

Quantity:

Bulk Order Anti-GABA-A-R-Beta2/3 Antibody

Product Details

Target

GABA-A-R-Beta2/3

Target Description

GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR-beta2/3 a is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR beta2/3 has been found to be expressed in brain at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

62-3G1

Isotype

IgG1

Applications

EM, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

GABRB3

Molecular Weight

55 kDa

Antigen

Fraction of bovine brain GABA-A receptor affinity purified against immobilized benzodiazepine Ro7-1986/1 (1988 de Blas et al J Neurosci PMID 2828565, 1988 Vitorica et al J Neurosci PMID 2828566). Target initially identified as a GABA-A receptor by: 1). rat brain immunocytochemistry staining pattern via light microscopy identical to that seen via ligand autoradiography with 3H-muscimol; 2). immunoprecipitation of 3H-muscimol-binding activity of the purified receptor complex; 3). recognition of ~57 kDa protein in immunoblots against crude rat brain membrane fractions Binding epitope identified to be within amino acids 26-40 (QSVNDPGNMSFVKET, extracellular N-terminus, 1992 Ewert et al Brain Res, PMID 1377081) of human GABA-A-R-Beta3 (also known as Gamma-aminobutyric acid receptor subunit beta-3, accession number P28472)

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Antibody Registry ID

AB_2315838

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Cross-reacts with GABA-A-R-Beta2 Does not cross-react with GABA-A-RBeta1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Gamma-aminobutyric acid receptor subunit beta-3 (GABA(A) receptor subunit beta-3)

UniProt Details

UniProt (Human): P28472
UniProt (Immunogen Species): P28472

Wei J, Graziane NM, Gu Z, Yan Z. (2015), 'DISC1 Protein Regulates γ-Aminobutyric Acid, Type A (GABAA) Receptor Trafficking and Inhibitory Synaptic Transmission in Cortical Neurons..' J Biol Chem. 10.1074/jbc.M115.656173.

Recover password

Create my account

Anti-GABA-A-R-Beta2/3 Antibody (62-3G1)

Available Conjugates

This antibody recognizes an epitope found in both GABA AR beta 2 and 3.

Anti-GABA-A-R-Beta2/3 Antibody
SKU: 75-363

Bulk Order Anti-GABA-A-R-Beta2/3 Antibody

Product Details

Related Products

Recently Viewed

Anti-GABA-A-R-Beta2/3 Antibody (62-3G1)

Available Conjugates

This antibody recognizes an epitope found in both GABA AR beta 2 and 3.

Anti-GABA-A-R-Beta2/3 Antibody SKU: 75-363

Bulk Order Anti-GABA-A-R-Beta2/3 Antibody

Product Details

Anti-GABA-A-R-Beta2/3 Antibody
SKU: 75-363