Anti-GABA-A-R-Beta2/3 Antibody (62-3G1)

Name: Anti-GABA-A-R-Beta2/3 Antibody
Brand: NeuroMab
SKU: 75-363
Price: 319.0 USD
Availability: InStock

10 Citations

Our Anti-GABA-A-R-Beta2/3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone 62-3G1. It detects bovine, chicken, human, mouse, and rat GABA-A-R-Beta2/3, and is purified by Protein A chromatography. It is great for use in EM, IHC, ICC, IP, WB.

Adult rat whole brain immunohistochemistry

Adult rat brain membrane immunoblot

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Applications

EM, ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-363

Product Details

Target

GABA-A-R-Beta2/3

Target Description

GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR-beta2/3 a is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR beta2/3 has been found to be expressed in brain at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

62-3G1

Isotype

IgG1

Applications

EM, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

GABRB3

Molecular Weight

55 kDa

Antigen

Fraction of bovine brain GABA-A receptor affinity purified against immobilized benzodiazepine Ro7-1986/1 (1988 de Blas et al J Neurosci PMID 2828565, 1988 Vitorica et al J Neurosci PMID 2828566). Target initially identified as a GABA-A receptor by: 1). rat brain immunocytochemistry staining pattern via light microscopy identical to that seen via ligand autoradiography with 3H-muscimol; 2). immunoprecipitation of 3H-muscimol-binding activity of the purified receptor complex; 3). recognition of ~57 kDa protein in immunoblots against crude rat brain membrane fractions Binding epitope identified to be within amino acids 26-40 (QSVNDPGNMSFVKET, extracellular N-terminus, 1992 Ewert et al Brain Res, PMID 1377081) of human GABA-A-R-Beta3 (also known as Gamma-aminobutyric acid receptor subunit beta-3, accession number P28472)

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Antibody Registry ID

AB_2315838

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
ICC: 1:250

Conjugate

Unconjugated

Specificity

Cross-reacts with GABA-A-R-Beta2 Does not cross-react with GABA-A-RBeta1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Gamma-aminobutyric acid receptor subunit beta-3 (GABA(A) receptor subunit beta-3)

UniProt Details

UniProt (Human): P28472
UniProt (Immunogen Species): P28472

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
PLA: Mouse
Western Blot: Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
33083769	2ug/ml	Verstraelen, P., et al. 2020. Systematic Quantification of Synapses in Primary Neuronal Culture. iScience, 101542.

Immunocytochemistry: Rat
PMID	Dilution	Publication
35312684	1:250	Schmerl, B., et al. 2022. The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses. PLoS Biology, e3001503.
26424793	1:250	Wei, J., et al. 2015. DISC1 Protein Regulates γ-Aminobutyric Acid, Type A (GABAA) Receptor Trafficking and Inhibitory Synaptic Transmission in Cortical Neurons.. Journal of Biological Chemistry, 27680-7.

PLA: Mouse
PMID	Dilution	Publication
33083769	not listed	Verstraelen, P., et al. 2020. Systematic Quantification of Synapses in Primary Neuronal Culture. iScience, 101542.

Western Blot: Mouse
PMID	Dilution	Publication
38201218	1:1000	Chen, M, et al. 2023. Brain Region Differences in α1- and α5-Subunit-Containing GABAA Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics. Cells, 14.
33192297	1:1000	Zhang, Y., et al. 2020. Enhanced prefrontal neuronal activity and social dominance behavior in postnatal forebrain excitatory neuron-specific Cyfip2 knock-out mice. . Frontiers in molecular neuroscience, p.201., 574947.
33083769	1ug/ml	Verstraelen, P., et al. 2020. Systematic Quantification of Synapses in Primary Neuronal Culture. iScience, 101542.
30797895	not listed	Dargaei, Z., et al. 2019. Alterations in hippocampal inhibitory synaptic transmission in the R6/2 mouse model of Huntington’s disease. . Neuroscience, 130-140.
30784587	1:500	Davenport, E.C., et al. 2019. Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition. Cell Reports, 2037-2051.

Western Blot: Rat
PMID	Dilution	Publication
29706582	1:500	Nguyen, Q.A., et al. 2018. The GABAA Receptor β Subunit Is Required for Inhibitory Transmission. Neuron, 718-725.

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Anti-GABA-A-R-Beta2/3 Antibody (62-3G1)

SKU: 75-363

Product Details

Product Specific References for Applications and Species

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