Our Anti-GABA-A-R-Beta2/3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone 62-3G1. It detects bovine, chicken, human, mouse, and rat GABA-A-R-Beta2/3, and is purified by Protein A chromatography. It is great for use in IHC, ICC.
GABA A receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR-beta2/3 a is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR beta2/3 has been found to be expressed in brain at the cell junction, synapse, postsynaptic cell membrane and cytoplasmic vesicle membrane.
Purified by Protein A chromatography
Fraction of bovine brain GABA-A receptor affinity purified against immobilized benzodiazepine Ro7-1986/1 (1988 de Blas et al J Neurosci PMID 2828565, 1988 Vitorica et al J Neurosci PMID 2828566). Target initially identified as a GABA-A receptor by: 1). rat brain immunocytochemistry staining pattern via light microscopy identical to that seen via ligand autoradiography with 3H-muscimol; 2). immunoprecipitation of 3H-muscimol-binding activity of the purified receptor complex; 3). recognition of ~57 kDa protein in immunoblots against crude rat brain membrane fractions Binding epitope identified to be within amino acids 26-40 (QSVNDPGNMSFVKET, extracellular N-terminus, 1992 Ewert et al Brain Res, PMID 1377081) of human GABA-A-R-Beta3 (also known as Gamma-aminobutyric acid receptor subunit beta-3, accession number P28472)
Bovine, Chicken, Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL550 Ex: 550 nm, Em: 575 nm
Cross-reacts with GABA-A-R-Beta2 Does not cross-react with GABA-A-RBeta1
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.