Anti-Fibrillarin (Nop1p) Antibody (38F3)

Name: Anti-Fibrillarin (Nop1p) Antibody
Brand: Biosensis
SKU: M-1372-250
Price: 327.0 USD
Availability: InStock

Our Anti-rRNA 2'O-methyltransferase fibrillarin (Nop1p) mouse monoclonal primary antibody detects C. elegans, drosophila, human, other mammals (predicted), rat, and yeast rRNA 2'O-methyltransferase fibrillarin (Nop1p), and is IgG. It is validated for use in ICC, WB.

A: Immunofluorescence staining of fibrillarin (Nop1p) expressed in nucleoli with M-1372-250 (green). Cells were double-immunolabelled with an antibody to neurofilament NF-H (red). Blue: cell nucleus, stained with DAPI.B: Western blot of whole yeast protein extract with M-1372-250 (left lane). Right lane: no primary antibody control.

$Left: Visualization of fibrillarin expression (green) in HeLa cells by Immunocytochemistry . Staining was performed with mouse anti-fibrillarin antibody diluted 1:10 from concentrated tissue culture media, and chicken antibody to vimentin (C-1409-50, 1:1,000, red). Blue: DAPI nuclear stain. The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments. Right: Western blot analysis of cell lysates with mouse antibody to fibrillarin (1:500, green). [1] protein standard, [2] C6 cytosol, [3] C6 nucleus, [4] HEK293 cytosol, [5] HEK293 nucleus, [6] NIH-3T3 cytosol and [7] NIH-3T3 nucleus. The band at 37 kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.$

A: Immunofluorescence staining of fibrillarin (Nop1p) expressed in nucleoli with M-1372-250 (green). Cells were double-immunolabelled with an antibody to neurofilament NF-H (red). Blue: cell nucleus, stained with DAPI.
B: Western blot of whole yeast protein extract with M-1372-250 (left lane). Right lane: no primary antibody control.

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Species Reactivity

Drosophila, Human, Rat, Yeast

Applications

ICC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1372-250

Volume/Size: 250 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

rRNA 2'O-methyltransferase fibrillarin (Nop1p)

Scientific Background

Fibrillarin is part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This means that suitably cross-reactive antibodies to Nop1p/fibrillarin such as this antibody can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archea tested to date. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.

Format

IgG

Clonality

Monoclonal

Clone

38F3

Isotype

IgG

Applications

ICC, WB

Host Species

Mouse

Molecular Weight

34 kDa

Immunogen

Yeast nuclear preparations. Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).

Immunogen Species

Yeast

Species Reactivity

Drosophila, Human, Rat, Yeast

Storage

Spin the vial briefly before opening it. Reconstitute in 250 uL sterile-filtered, room temperature, ultrapure water. Let the vial rehydrate for 5 minutes. Mix gently with a pipette tip to help the redissolution of the material. Centrifuge to remove any insoluble material. The final solution will contain no preservatives. A sterile technique is recommended.After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Concentrated cell culture supernatant.

Buffer

Lyophilized hybridoma cell culture media with sodium azide

Dilution Ranges

WB: 1:500-1:10000 (see notes)
ICC: 1:500-1:5000 (see notes)

Application Details

A dilution of 1:2000 (cell lysates) to 1:10000 (nuclear fractions) is recommended for WB of yeast protein samples followed by chemiluminescent detection (ECL). For other non-ECL WB methods, a dilution of 1:1000 to 1:5000 is recommended. A dilution of 1:500 is recommended for ECL WB on mammalian fibrillarin. A dilution of 1:1000 to 1:5000 is recommended for ICC on yeast cells and 1:500 for mammalian cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB. This clone was selected because it is specific for the ~34 kDa Fibrillarin. It stains a single band on Western blotting and shows a clear and strong punctate staining of yeast nuclei. It can therefore be used as a marker for nucleoli in a wide variety of species. Human, rat, Drosophila, S. pombe, C. elegans and and S. pombe. Predicted to react with other mammalian tissues.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; FBL; FIB1; FLRN; Nop1p

UniProt ID

P15646

Shipping

25°C (ambient)

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Anti-Fibrillarin (Nop1p) Antibody (38F3)

SKU: M-1372-250

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