Anti-Glial fibrillary acidic protein (GFAP) Antibody (5C10)
Our Anti-Glial fibrillary acidic protein (GFAP) mouse monoclonal primary antibody detects avian (predicted), bovine, human, mouse, other mammals (predicted), pig, and rat Glial fibrillary acidic protein (GFAP), and is IgG. It is validated for use in ICC, IHC-Frozen, WB.
![Mixed neuron-glial cultures stained with Mouse monoclonal antibody to Glial Fibrillary Acidic Protein [5C10] M-1375-100 (red) and chicken polylclonal antibody to neurofilament L C-1390-50 (green). The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA.](http://www.antibodiesinc.com/cdn/shop/files/m-1375-100-ihc_120x120.jpg?v=1759274343)
![Right: Detection of GFAP expression in rat cerebellum by Immunohistochemistry with mouse antibody to GFAP (1:1,000, green), and co-stained with rabbit antibody to NF-L (R-1392-50, 1:2,000, red). IHC Method: Following transcardial perfusion with 4% PFA, brain was post-fixed for 24 hours, cut to 45 um sections, and free-floating sections were stained. Clone 5C10 stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes. Right: Western blot analysis of whole tissue lysates using mouse antibody to GFAP (1:2,000, green). [1] protein standard , [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 50 kDa corresponds to the GFAP protein.](http://www.antibodiesinc.com/cdn/shop/files/m-1375-100-ihc-wb_120x120.jpg?v=1759274343)
Mixed neuron-glial cultures stained with Mouse monoclonal antibody to Glial Fibrillary Acidic Protein [5C10] M-1375-100 (red) and chicken polylclonal antibody to neurofilament L C-1390-50 (green). The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA.
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SKU: M-1375-100
Product Details
Glial fibrillary acidic protein (GFAP)
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
IgG
Monoclonal
5C10
IgG1
ICC, IHC, WB
Mouse
50 kDa
Purified GFAP from porcine spinal cord
Pig
Bovine, Human, Mouse, Pig, Rat
Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Lyophilized
Protein G purified
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
WB: 1:5000
IHC: 1:500-1:1000
ICC: 1:500-1:1000
IHC: 1:500-1:1000
ICC: 1:500-1:1000
Unconjugated
The specificity of this antibody has been confirmed by WB. Human, rat, mouse, bovine, Porcine. Predicted to react with other mammalian and avian species.
For research use only.
United States
12 months after date of receipt (unopened vial).
Astrocyte; Glial fibrillary acidic protein; GFAP;
25°C (ambient)