Anti-Cellular oncogene fos (c-Fos) Antibody

Name: Anti-Cellular oncogene fos (c-Fos) Antibody
Brand: Biosensis
SKU: S-045-50
Price: 337.0 USD
Availability: InStock

Our Anti-Cellular oncogene fos (c-Fos) sheep polyclonal primary antibody detects hamster, human, rabbit, and rat Cellular oncogene fos (c-Fos), and is affinity-purified. It is validated for use in IHC-Frozen, WB.

c-Fos was induced in the hypothalamic arcuate nucleus of an adult male Wistar rat with i.p. injection of N-methyl-D-aspartate. The brain was fixed by transcardiac perfusion of the rat with formaldehyde (4%). cFos immunoreactivity was detected on floated cryo-sections of the hypothalamic arcuate nucleus with the S-045-50 primary antibody (1:5000) using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

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Species Reactivity

Hamster, Human, Rabbit, Rat

Applications

IHC, WB

Host Species

Sheep

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Data Sheet

SKU: S-045-50

Volume/Size: 50 µg

Variant

Price:

$337.00

Quantity:

Product Details

Target

Cellular oncogene fos (c-Fos)

Scientific Background

FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Sheep

Immunogen

A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.

Immunogen Species

Human

Species Reactivity

Hamster, Human, Rabbit, Rat

Storage

Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized product at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized from a solution containing 3% Trehalose in PBS pH 7.4

Dilution Ranges

IHC: 1-5 µg/mL

Application Details

IHC on PFA fixed, frozen tissues. Not yet tested on formalin fixed, paraffin embedded tissue but expected to react. Affinity purified antibody has strong signal. A concentration of 1-5 µg/mL is recommended most uses with short (1-8 hour) incubations. If using enhanced brightfield or amplified detection methods dilutions and long primary antibody incubations dilutions will need to be increased substantially to inhibit background staining.

Not recommended for Western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting.

Conjugate

Unconjugated

Specificity

This antiserum shows a high level of specificity for cFOS confirmed by immunohostochemistry. This antiserum is known to react with rat, rabbit and hamster cFOS.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Proto-oncogene protein cFOS; c-FOS

UniProt ID

P01100

Shipping

25°C (ambient)

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Anti-Cellular oncogene fos (c-Fos) Antibody

SKU: S-045-50

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