Anti-Beta-nerve growth factor (beta-NGF) Antibody
Our Anti-Beta-nerve growth factor (beta NGF) sheep polyclonal primary antibody detects avian, human, mouse, and rat Beta-nerve growth factor (beta NGF), and is whole serum. It is validated for use in ELISA, IHC-Frozen, Neutralization, WB.
SKU: S-050-250
Product Details
Beta-nerve growth factor (beta NGF)
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Whole serum
Polyclonal
Mixed
ELISA, IHC, Neutralize, WB
Sheep
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Mouse
Human, Mouse, Rat
Spin vial briefly before opening. Reconstitute in 250 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Lyophilized
WB: 1:1000-1:5000
IHC: 1:1000-1:5000
ELISA: 1:15000
IHC: 1:1000-1:5000
ELISA: 1:15000
For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro.
Unconjugated
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
For research use only.
United States
12 months after date of receipt (unopened vial).
Beta-nerve growth factor
25°C (ambient)