Anti-Cav3.2 Ca2+ Channel Antibody (N55/10)

Name: Anti-Cav3.2 Ca2+ Channel Antibody
Brand: NeuroMab
SKU: 75-095
Price: 337.0 USD
Availability: InStock

Our mouse Cav3.2 Ca2+ channel monoclonal antibody from NeuroMab is produced in-house from clone N55/10. It detects human, mouse, rat, and tree shrew Cav3.2 Ca2+ channel and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA.

Stable cell immunoblot: extracts of HEK cells stably-expressing Flag-tagged Cav3.2, Cav3.1 or untagged Kv2.1 plasmid and probed with N55/10 TC supe (left) or Rabbit anti-Flag (right).

Immunohistochemistry of coronal brain sections from WT and Cav3.2 KO mice. Reproduced with permission from Mala Shah (University College London, England, UK) and Nature Neuroscience (2011, Huang et al., PMID 21358644).

Stable cell immunoblot: extracts of HEK cells stably-expressing Flag-tagged Cav3.2, Cav3.1 or untagged Kv2.1 plasmid and probed with N55/10 TC supe (left) or Rabbit anti-Flag (right).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat, Tree Shrew

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-095

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Cav3.2 Ca2+ channel

Scientific Background

Voltage-dependent T-type calcium channel subunit alpha-1H or Cav3.2 Ca2+ channel is encoded by the gene CACNA1H. Cav3.2 is a pore forming alpha subunit of T voltage-sensitive calcium channels and gives rise to T-type calcium currents. (transient opening calcium channels). These channels facilitate entry of calcium ions into excitable cells are involved in a variety of calcium dependent processes, including neurotransmitter release, muscle contraction, and gene expression. Cav3.2 is expressed in kidney, liver, and heart and brain.as a membrane bound protein. Diseases associated with this gene include Hyperaldosteronism and Epilepsy.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N55/10

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

CACNA1H

Molecular Weight

260 kDa

Immunogen

Fusion protein amino acids 1019-1293 (cytoplasmic loop between repeat II and repeat III) of human Cav3.2 (accession number O95180), epitope mapped to amino acids 1179-1192 (AEDGRAAPGPRATP) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat, Tree Shrew

Antibody Registry ID

AB_2069551

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.22

Conjugate

Unconjugated

Specificity

Does not cross-react with Cav3.1

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Voltage-dependent T-type calcium channel subunit alpha-1H (Low-voltage-activated calcium channel alpha1 3.2 subunit) (Voltage-gated calcium channel subunit alpha Cav3.2)

UniProt ID

O95180

Shipping

Shipped on ice packs

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Anti-Cav3.2 Ca2+ Channel Antibody (N55/10)

SKU: 75-095

Product Details

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