Anti-FGF14/FHF4 Antibody (N56/21)

Name: Anti-FGF14/FHF4 Antibody
Brand: NeuroMab
SKU: 75-096
Price: 337.0 USD
Availability: InStock

Our mouse FGF14/FHF4 monoclonal antibody from NeuroMab is produced in-house from clone N56/21. It detects human, mouse, non-human primate, and rat FGF14/FHF4 and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA.

N56/21 immunofluorescence staining of cerebellar cortex from FGF14 wild-type (left) and knockout (right) mice. ml: molecular layer; pc: Purkinje cell layer; igl: internal granule cell layer; wm: white matter.

Double immunofluorescence staining of cultured rat hippocampal neurons with N56/21 (red) and MAP2 (green). Images courtesy of David Ornitz (Washington University SOM).

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged FGF14b or untagged Kv2.1 plasmid and probed with N56/21 (left) or 19E10 (right) TC supe.

Knockout mice. ml: molecular layer; pc: Purkinje cell layer; igl: internal granule cell layer; wm: white matter.

N56/21 immunofluorescence staining of cerebellar cortex from FGF14 wild-type (left) and knockout (right) mice. ml: molecular layer; pc: Purkinje cell layer; igl: internal granule cell layer; wm: white matter.

Click on image to zoom

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-096

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

FGF14/FHF4

Scientific Background

Fibroblast growth factor 14, FHF4 or FGF14 is encoded by FGF14. FHF4 is a member of the fibroblast growth factor homologous factors family (a subfamily of fibroblast growth factors.). FHFs have a role in regulation of voltage fated sodium channels (Nav channels) and are thought to be involved in nervous system development. FGF14 in found in both the hippocampus and the cerebellum localizes to the axon initial segment. Diseases associated with FGF14 include Spinocerebellar Ataxia and Autosomal Dominant Cerebellar Ataxia.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N56/21

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Fgf14

Molecular Weight

28 kDa

Immunogen

Fusion protein amino acids 1-252 (full-length) of mouse FGF14b (accession number O89096) produced recombinantly in E. Coli

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Non-Human Primate, Rat

Antibody Registry ID

AB_2104060

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.23

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Cross-reacts with FGF14a and FGF14b Does not cross-react with FGF11, FGF12 or FGF13

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Fibroblast growth factor (FGF)

UniProt ID

O89096

Shipping

Shipped on ice packs

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Anti-FGF14/FHF4 Antibody (N56/21)

SKU: 75-096

Product Details

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