Our Anti-Cav3.2 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N55/10. It is KO validated, detects human and mouse Cav3.2 Ca2+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC.
Cav3.2 Ca2+ channel
Voltage-dependent T-type calcium channel subunit alpha-1H or Cav3.2 Ca2+ channel is encoded by the gene CACNA1H. Cav3.2 is a pore forming alpha subunit of T voltage-sensitive calcium channels and gives rise to T-type calcium currents. (transient opening calcium channels). These channels facilitate entry of calcium ions into excitable cells are involved in a variety of calcium dependent processes, including neurotransmitter release, muscle contraction, and gene expression. Cav3.2 is expressed in kidney, liver, and heart and brain.as a membrane bound protein. Diseases associated with this gene include Hyperaldosteronism and Epilepsy.
Purified by Protein A chromatography
Fusion protein amino acids 1019-1293 (cytoplasmic loop between repeat II and repeat III) of human Cav3.2 (accession number O95180), epitope mapped to amino acids 1179-1192 (AEDGRAAPGPRATP) produced recombinantly in E. Coli
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
FL650 Ex: 655 nm, Em: 676 nm
Does not cross-react with Cav3.1
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.