Anti-Myristoylated alanine rich C kinase substrate (MARCKS) Antibody
Our Anti-Myristoylated alanine rich C kinase substrate (MARCKS) rabbit polyclonal primary antibody detects human Myristoylated alanine rich C kinase substrate (MARCKS), and is affinity purified. It is validated for use in ICC, WB.
![Left: Analysis of MARCKS expression (red) in HeLa cells by Immunocytochemistry. MARCKS protein was detected with rabbit antibody to MARCKS at 1:1,000 dilution. Cells were co-stained with a mouse anti-beta tubulin antibody (green). Blue: DAPI nuclear stain. The MARCKS antibody recognizes protein localized in the plasma membrane and cytoplasm. Right: Western blot analysis of MARCKS expression (green) in various cell line lysates using rabbit anti-MARCKS antibody (1:1,000). [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, [7] C6. The blot was probed simultaneously with mouse antibody to GAPDH (M-1376-250, red, 1:5,000). The strong band at 80 kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells (lane 5), but no reactivity is seen on rodent cells (lanes 6 and 7). The GAPDH antibody used as a loading control reveals a single band at 37 kDa in all preparations.](http://www.antibodiesinc.com/cdn/shop/files/r-1380-50-ihc-wb_120x120.jpg?v=1759274407)
Image of mixed neuron/glial cultures stained with Rabbit polyclonal antibody to Myristoylated alanine rich C kinase substrate (MARCKS) R-1380-50 (red) and Chicken polyclonal antibody to MAP2 C-1382-50. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.
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