Anti-Ankyrin-G (Staining) Antibody (N106/65)

Name: Anti-Ankyrin-G (Staining) Antibody
Brand: NeuroMab
SKU: 75-147
Price: 394.0 USD
Availability: InStock

Our mouse Ankyrin-G (Staining) monoclonal antibody from NeuroMab is produced in-house from clone N106/65. It detects human, mouse, and rat Ankyrin-G (Staining) and is purified by Protein A chromatography. Works in IHC, ICC, ELISA, EM.

Immunofluorescence labeling of axon initial segments in adult rat cortex with N106/65 TC supe (red) and Hoechst nuclear stain (blue).

Representative images showed the high expression level of FKBP12 (green) in axonal hillock/axon initial segment (labeled by anti–ankyrin-G antibody, cat. 75-147; bright blue) under basal conditions whereas FKBP12 translocated to soma and dendrites when neurons bear tau aggregation. Image from publication CC-BY-4.0. PMID:36724228

Immunolabeling of mouse cultured neurons identifying axonal segments with the labeling of Ankyrin G (Cat. 75-147). Image in publication. PMID: 39626668. CC-BY-4.0.

Immunofluorescence labeling of axon initial segments in adult rat cortex with N106/65 TC supe (red) and Hoechst nuclear stain (blue).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, EM, ICC, IHC

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 75-147

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ankyrin-G (Staining)

Scientific Background

Ankyrin-G (ankyrin 3) is a member of the Ankyrin family which consists of Ankyrin R (1), Ankyrin B (2) and Ankyrin G (3) all of which are expressed in the nervous system. Ankyrins are structural proteins that aid in the attachment of integral membrane proteins with the spectrin-actin cytoskeleton, and thus targets voltage-gated sodium channels, potassium channels, cell adhesion molecules and others at the nodes of Ranvier and axonal initial segments (AIS). Variants in Ankyrin G have been linked to bipolar disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N106/65

Isotype

IgG2b

Applications

ELISA, EM, ICC, IHC

Host Species

Mouse

Gene Name

ANK3

Molecular Weight

<200 kDa

Immunogen

Fusion protein ~1000 amino acids of Ankyrin-G (accession number E9PE32) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10675130

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.74

Dilution Ranges

IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with Ankyrin-B

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Ankyrin-3 (ANK-3) (Ankyrin-G)

UniProt ID

E9PE32

Shipping

Shipped on ice packs

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Anti-Ankyrin-G (Staining) Antibody (N106/65)

SKU: 75-147

Product Details

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