Anti-5-Hydroxymethylcytidine Antibody (N297/59)

Name: Anti-5-Hydroxymethylcytidine Antibody
Brand: NeuroMab
SKU: 75-268
Price: 337.0 USD
Availability: InStock

Our mouse 5-hydroxymethylcytidine monoclonal antibody from NeuroMab is produced in-house from clone N297/59. It detects n/a 5-hydroxymethylcytidine and is purified by Protein A chromatography. Works in IP, ELISA.

Serial dilutions of BSA-conjugated modified and unmodified nucleotides dotted onto membrane and probed with N297/59 TC supe (left) and a rabbit polyclonal control (right).

Click on image to zoom

Applications

ELISA, IP

Host Species

Mouse

Conjugates and Formulations

Unconjugated

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-268

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

5-hydroxymethylcytidine

Scientific Background

5-hydroxymethylcytidine is a DNA pyrimidine nitrogen base derived from cytosine. It is potentially important in epigenetics. In mammals and is thought to be important in gene regulation. 5-hydroxymethylcytidine can be generated by oxidation of 5-methylcytosine, a reaction mediated by the Tet family of enzymes. 5-hydroxymethylcytidine is found in many tissue types.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N297/59

Isotype

IgG2a

Applications

ELISA, IP

Host Species

Mouse

Gene Name

N/A

Immunogen

Synthetic nucleotide of 5-hydroxymethylcytidine.

Antibody Registry ID

AB_11001670

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Reacts preferentially with 5-hydroxymethylcytidine than with 5-methylcytidine

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

UniProt ID

N/A

Shipping

Shipped on ice packs

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Anti-5-Hydroxymethylcytidine Antibody (N297/59)

SKU: 75-268

Product Details

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