Serial dilutions of BSA-conjugated modified and unmodified nucleotides dotted onto membrane and probed with N297/59 TC supe (left) and a rabbit polyclonal control (right).
Bulk Order Anti-5-Hydroxymethylcytidine Antibody
5-hydroxymethylcytidine is a DNA pyrimidine nitrogen base derived from cytosine. It is potentially important in epigenetics. In mammals and is thought to be important in gene regulation. 5-hydroxymethylcytidine can be generated by oxidation of 5-methylcytosine, a reaction mediated by the Tet family of enzymes. 5-hydroxymethylcytidine is found in many tissue types.
Purified by Protein A chromatography
Synthetic nucleotide of 5-hydroxymethylcytidine.
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Reacts preferentially with 5-hydroxymethylcytidine than with 5-methylcytidine
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.