Monoclonal Antibodies

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Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with TrpC4 and probed with N77/15 TC supe.Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.Transfected cell immunoblot: extracts of rat brain membranes (RBM) and COS cells transiently transfected with Flag-tagged GIT1 and GIT2 and untagged Kv2.1 plasmids and probed with N83/48 (left) and K89/41 (right) TC supe and with anti-Flag antibody (middle).
Adult rat hippocampus immunohistochemistry.Adult rat whole brain immunohistochemistry.
Adult rat brain membrane immunoblot.Immunoblot versus membranes from postnatal day 8 wild-type (wt) and ADAM22-knockout (ko) mice and probed with N46/30 (left) and N57/2 (right). Data courtesy of Dies Meijer, Erasmus University Rotterdam.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2 NGL-3 or untagged Kv2.1 plasmid and probed with N51/6 (left) or K89/41 (right) TC supe.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myc-tagged NGL-1, NGL-2, NGL-3 or untagged Kv2.1 plasmid and probed with N49A/21 (left and middle) or K89/34 (right) TC supe.
Immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with N449/73 (left) and N52A/42 (right) TC supe.Adult rat brain membrane immunoblot.
Adult rat brain membrane immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against extracts of COS cells transiently transfected with GFP-tagged zebrafish VSP (DrVSP) or untagged Xenopus (XtVSP, XlVSP and XlVSP2), sea squirt (CiVSP), chicken VSP (GgVSP) or Kv2.1 plasmid probed with N432/63 (left) or N52A/42 (right) TC supe.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Immunoblot against extracts of COS cells transiently transfected with untagged GlyRAlpha3L, GlyRAlpha3K or Kv2.1 plasmid probed with N424/45 (left) or K89/34 (right) TC supe.
Immunoblot against extracts of COS cells transiently transfected with untagged ANO5/TMEM16E, GFP- tagged ANO6/TMEM16F or untagged Kv2.1 plasmid probed with N421A/85 (left) or N86/38 (right) TC supe.
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with untagged TRAK1, GFP-tagged TRAK2 or untagged Kv2.1 plasmid probed with N389/9 (left) or N52A/42 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Immunoblot against crude membranes from adult rat brain (RBM) and wild-type (WT) and Ankyrin-R knockout (KO) mouse brains probed with N388A/10 (left) and K58/35 (right) TC supe. Mouse brains courtesy of Matthew Rasband (Baylor College of Medicine.
Immunoblot against extracts of COS cells transiently transfected with untagged TRAK1, GFP-tagged TRAK2 or untagged Kv2.1 plasmid probed with N390/43 (left) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
adult rat Whole brain immunohistochemistry.Adult rat hippocampus immunohistochemistry.
Immunoblot against extracts of COS cells transiently transfected with Myc-tagged Kir6.1, HA-tagged Kir6.2 or untagged Kv2.1 plasmid probed with N363/71 (left) or N52A/42 (right) TC supe.
Immunofluorescence staining of a node of Ranvier in adult rat sciatic nerve with N343/26 (green) and bIV-spectrin rabbit polyclonal (red). Image courtesy of Kae-Jiun Chang and Matt Rasband (Baylor College of Medicine.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged LRRK1, LRRK2 or untagged Kv2.1 plasmid and probed with N341/12 (left) or N241A/34 (right) TC supe.Immunoblot versus LRRK1-immunoprecipitated extracts from WT and LRRK1 KO mouse heart probed with N341/12 TC supe. Data courtesy of Paul Davies and Dario Alessi (University of Dundee, Scotland, UK.
Immunoblot versus extracts of heart tissue from WT and SUR2 KO mice probed with N323A/31 TC supe. Data courtesy of William Coetzee and Margaret Rice, New York University SOM.
Serial dilutions of BSA-conjugated modified and unmodified nucleotides dotted onto membrane and probed with N297/59 TC supe (left) and a rabbit polyclonal control (right).
Immunoblot versus crude membrane preparations from adult rat brain (RBM) and Brevican wild-type (WT) and knockout (KO) mice and probed with N294A/6 (left) and N52A/42 (right) TC supe. Mouse brain samples courtesy of Matthew Rasband, Baylor College of Medicine.
Immunohistochemistry with antigen retrieval via citrate pretreatment.Adult rat hippocampus immunohistochemistry under standard protocol.

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