Anti-CA19-9/Sialyl Lewis A (sLeA) Antibody (CA19-9-203)

Name: Anti-CA19-9/Sialyl Lewis A (sLeA) Antibody
Brand: Antibodies Incorporated
SKU: MM-1003
Price: 337.0 USD
Availability: InStock

Our mouse CA19-9/Sialyl Lewis A (sLeA) monoclonal antibody from Antibodies Incorporated is produced in-house from clone CA19-9-203. It detects human CA19-9/Sialyl Lewis A (sLeA) and is purified by Protein A chromatography. Works in IHC, ICC, WB, ELISA.

Immunofluorescent staining of SW1116 cells using 5 ug/mL mouse monoclonal anti-Sialyl Le (a) (19-9) antibody (MM-1003) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. Anti-Sialyl-Le stains the membrane of SW1116 cells.

Western blotting of SW1116 cell lysate using 0.2 ug/mL, 1 ug/mL, or 5 ug/mL Antibodies Incorporated mouse monoclonal anti-Sialyl Le (a) (19-9) antibody (MM-1003). MM-1003 mouse anti-Sialyl Le (a) (19-9) antibody recognizes endogenous Sialyl Le (a) (19-9) at 125 kDa.

Immunofluorescent staining of SW1116 cells using 5 ug/mL mouse monoclonal anti-Sialyl Le (a) (19-9) antibody (MM-1003) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. Anti-Sialyl-Le stains the membrane of SW1116 cells.

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Species Reactivity

Human

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: MM-1003

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

CA19-9/Sialyl Lewis A (sLeA)

Scientific Background

CA19-9/ Sialyl Lewis a (sialyted lacto-N-fucopentose) (α-NeuNAc-(2→3)-β-D-Gal-(1→3)-(α-L-Fuc-[1→4])-D-GlcNAc, 3′-SLea) corresponds to blood group antigens. In normal tissue Sialyl Lewis a is present in ductal epithelium of the breast, kidney and salivary glands. This antigen is expressed is increased in all gastrointestinal carcinomas (stomach, intestine and pancreas) (1) While it is not useful to diagnose particular forms of cancer, specifically pancreatic cancer, it may be useful to monitor the results of treatment and to determine whether the disease may be recurring (3) Other causes for elevated CA19-9 levels include Mirizzi’s syndrome and other hepatobiliary diseases (2) In patients that do not have the Lewis antigen blood type (about 5% of the population), CA19-9 is not elevated in pancreatic cancer even with large tumors because they have a deficiency of fucosyltransferase that is needed to produce CA19-9 (1).

Format

Purified by Protein A Chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

CA19-9-203

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Molecular Weight

120-400 kDa

Immunogen

Purified human mucin from ovarian cyst

Immunogen Species

Human

Species Reactivity

Human

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000 -1:5000
IHC: 1:200
ICC: 1:200

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

CA19-9; Sialyl Lewis A; sLeA

Shipping

Shipped on ice packs

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Anti-CA19-9/Sialyl Lewis A (sLeA) Antibody (CA19-9-203)

SKU: MM-1003

Product Details

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