Our mouse Cytokeratins 1-6, 8, 10, 13-16, 18, 19 monoclonal antibody from Antibodies Incorporated is produced in-house from clone Multiple clones. It detects human Cytokeratins 1-6, 8, 10, 13-16, 18, 19 and is purified by Protein A chromatography. Works in IHC, ICC, WB, ELISA.
Immunofluorescent staining of MCF7 cells using 1 ug/mL mouse monoclonal anti-Cytokeratin-cocktail antibody (MM-1012) (green). The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI mounting medium (Cat. AR-6501). DAPI nuclear stain (blue) shows cell nuclei. Anti-Cytokeratin-cocktail stains the cytoskeleton of MCF7 cells.
Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue. There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46). This antibody recognizes cytokeratins CK1 (67), CK2 (65.5), CK3 (64), CK4 (59) CK5 (58), CK6 (56), CK8 (52.5), CK10 (56.5), CK13 (54) CK14 (50), CK15 (50), CK16 (48), CK18 (45), CK 19 (40)
Purified by Protein A Chromatography
1 mg/mL
Monoclonal
Multiple clones
IgG1
ELISA, ICC, IHC, WB
Mouse
KRT1-6, 8, 10, 13-16, 18, 19
40-67 kDa
Human epidermal keratin
Human
Human
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Bulk Order Anti-Cytokeratin Cocktail Antibody
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