Fluorescent Cell Proliferation Assay

1 Citation

An oxidation/reduction-based reagent quantitatively measures the reducing power of living cells. The cell-permeable reagent is blue in color, but non-fluorescent. When reduced by viable cells, it fluoresces red. Read in a fluorescent plate reader.

Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.
Figure 2. PBMC proliferation in response to mitogen Concanavalin A (Con-A) stimulation measured using Fluorescent Cell Proliferation Assay. PBMC at 15,000 cells were cultured for 3 days in the presence of titrated amounts of Con A and tested for proliferation using the Fluorescent Cell Proliferation Assay. Dye reagent (1/10 volume) was added to the cultured cells and incubated at 37°C for 2h. Fluorescence was detected at Ex: 530 nm and Em: 590 nm.
Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.
Figure 1. Staurosporine induced Jurkat cell viability assay. Titrated doses of staurosporine were added into seeded Jurkat cells (10,000 cells/well) in 96-well black opaque tissue culture plates and incubated at 37°C, 10% CO2 for 24h. Dye reagent (1/10 volume) was added into the cultured cells and incubated for 3h and fluorescence was detected at Ex: 530 nm and Em: 590 nm.
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Data Sheet
Protocol

SKU: INDBLU100-4P

Size: 1000 Tests (with 10 Black Clear Bottom Plates)
Price:
$343.00
Ships: 1-2 business days
INDBLU100-3: 500 Tests
  • Part # 4024: IndoBluTM Reagent (2-8C)
  • Optional: 96-Well Clear Bottomed Black Plate for Fluorescent Readout

    • INDBLU100-3P: 500 Tests (with 5 Black Clear Bottom Plates)
  • Part # 4024: IndoBluTM Reagent (2-8C)
  • 96-Well Clear Bottomed Black Plate for Fluorescent Readout x 5

    • INDBLU100-4: 1000 Tests
  • Part # 4024: IndoBluTM Reagent x 2 (2-8C)
  • Optional: 96-Well Clear Bottomed Black Plate for Fluorescent Readout

    • INDBLU100-4P: 1000 Tests (with 10 Black Clear Bottom Plates)
  • Part # 4024: IndoBluTM Reagent x 2 (2-8C)
  • 96-Well Clear Bottomed Black Plate for Fluorescent Readout x 10

    • Product Specific References

    PMID Publication
    25055824Coaxum, S. D., et al. 2014. Epidermal growth factor-induced proliferation of collecting duct cells from Oak Ridge polycystic kidney mice involves activation of Na+/H+ exchanger. Am J Physiol Cell Physiol, C554-60.

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