Fluorescent Lactate Detection Kit

Name: Fluorescent Lactate Detection Kit
Brand: ImmunoChemistry Technologies
SKU: FLLACT100-2
Price: 442.0 USD
Availability: InStock

This kit provides a reliable, sensitive fluorometric method for the quantification of lactate in biological samples such as serum, plasma, urine, and tissue extracts. The assay plate can be incubated and read in 15-30 min.

Figure 2. Typical standard curve. A new curve must be generated each time the assay is run.

Figure 3. At reduced glutathione concentrations higher than 0.2 mM, detection reagent oxidation results from side chain reaction between GSH and HRP. This could cause aberrant readings. To minimize this interference it is recommended to add N-Ethylmaleimide (NEM) at 40 mM to the reaction (4 μM of lactate was used here).

Figure 1. Assay principle

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Protocol

Safety Data Sheet

SKU: FLLACT100-2

Size: 100 Tests

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Price:

$442.00

Quantity:

Ships: 1-2 business days

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Scientific Background

Lactate is an intermediate product of carbohydrate metabolism. Of the two forms of Lactate, D- and L-, the L-lactate is the predominant isomer found in biological systems. L-lactate is formed during the anaerobic glycolysis by conversion of pyruvate to L-lactate by lactate dehydrogenase. Lactate level is an indicator for tissue oxygen demand and utilization. Abnormally high lactate levels are associated with diseases such as diabetes and lactate acidosis.

Our lactate assay is a lactate oxidase-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and tissue extracts. In the assay, lactate oxidase (LOX) catalyzes the oxidation of L-lactate to pyruvate, along with the concomitant reduction of hydrogen peroxide (H2O2). The detection utilizes a non-fluorescent detection reagent, which is oxidized in the presence of horseradish peroxidase and LOX to produce its fluorescent analog.

Method of Analysis

Fluorescence plate reader

Usage Statement

For research use only. Not for use in diagnostic procedures.

Country of Origin

United States

Storage

2-8 °C

Shipping

Ships overnight (domestic), International Priority Shipping

Part # 7022: Lactate Standard 4mM, 500µl (2-8C)

Part # 6004: Horseradish Peroxidase, 18.9 Units (2-8C)

Part # 3011: 5X Reaction Buffer, 25 mL (2-8C)

Part # 4026: Detection Reagent, 1 Vial (-20C)

Part # 6025: Reaction Enzyme Mix, 1 Vial - 1.1mL (-20C)

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Fluorescent Lactate Detection Kit

SKU: FLLACT100-2

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