Fluoro Catalase Activity Detection Kit by Cell Technology

Name: Fluoro Catalase Activity Detection Kit by Cell Technology
Brand: ImmunoChemistry Technologies
SKU: FLOCAT100-3
Price: 352.0 USD
Availability: InStock

A sensitive, one-step, no wash assay for the sensitive monitoring of catalase activity by measuring residual hydrogen peroxide substrate. A non-fluorescent detection reagent generates a fluorescent signal in the presence of peroxidase and resorufin.

Figure 2. The reaction contained 20mM hydrogen peroxide (final) per well and the indicated amounts of catalase. The reaction was incubated for 30 minutes at room temperature. Reaction cocktail was added to each well and the reaction incubated for another 10 minutes in the dark at room temperature. Fluorescence was measured at excitation 530nm and emission 590nm. The graphs show the net fluorescence change: Negative Control fluorescence (no catalase) minus Sample (Cat. alase) fluorescence.

Figure 1. Assay principle

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Data Sheet

Protocol

Safety Data Sheet

SKU: FLOCAT100-3

Size: 500 Tests

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Price:

$352.00

Quantity:

Ships: 1-2 business days

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Scientific Background

Catalase is an antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Catalase is ubiquitously expressed in mammalian and non‐mammalian aerobic cells containing the cytochrome system. The enzyme has been isolated from various sources, including bacteria and plant cells. Catalase activity varies greatly from tissue to tissue. Highest activity is seen in liver and kidney, while lowest activity is seen in connective tissue. In eukaryotic cells, catalase in concentrated in organelles called peroxisomes.

The production of hydrogen peroxide in eukaryotic cells is an end product result of various oxidases and superoxide dismutase reactions. Accumulation of H2O2 can result in cellular damage through oxidation of proteins, DNA and lipids thus resulting in cell death and mutagenesis. Hydrogen peroxide’s role in oxidative stress related diseases has been widely studied.

The Fluoro Catalase detection kit is a sensitive assay that utilizes a non‐fluorescent detection reagent to measure H2O2 substrate left over from the catalase reaction.

Method of Analysis

Fluorescence plate reader

Usage Statement

For research use only. Not for use in diagnostic procedures.

Country of Origin

United States

Storage

2-8 °C

Shipping

Ships overnight (domestic), International Priority Shipping

3021: 5X Reaction Buffer pH 7.4, 25mL (2-8C)

4010: Detection Reagent, 1 Vial (2-8C - Undiluted; Diluted: -20C (Aliquot in Single Use Vials)

6009: Horseradish Peroxidase, 1 Vial (2-8C - Undiluted; Diluted: -20C)

3022: Hydrogen Peroxide, 1 Vial (2-8C)

6008: Catalase Enzyme, 1 Vial (2-8C (Crystalline Suspension)

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Fluoro Catalase Activity Detection Kit by Cell Technology

SKU: FLOCAT100-3

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